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Genetic Predictors of Polybrominated biphenyl (PBB) Elimination

ABSTRACT: Genetic Predictors of Polybrominated biphenyl (PBB) Elimination

PROVIDER: PRJNA553008 | ENA |

REPOSITORIES: ENA

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Genetic Predictors of Polybrominated biphenyl (PBB) Elimination

Project description:Genetic Predictors of Polybrominated biphenyl (PBB) Elimination

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Exposure to polybrominated biphenyl (PBB) associates with DNA methylation differences across the genome

Project description:Polybrominated biphenyl (PBB) is an endocrine-disrupting compound which was accidently added to the food supply in Michigan in the 1970’s. Highly exposed individuals and their children have numerous health problems, though the underlying mechanism behind these health problems remains unknown. Other endocrine-disrupting compounds have been linked to epigenetic differences, but no epigenetic studies have been done for PBB. In this study, DNA from the blood of individuals with PBB exposure from the Michigan PBB Registry was interrogated with the MethylationEPIC BeadChip (N = 659). Associations between each of the ~850,000 CpG sites and current, serum PBB levels were tested with a linear regression that controlled for age, sex, and cell type proportions. We show that exposure to PBB is associated with differences in epigenetic marks that suggest that it is acting similarly to estrogen and is associated with dysregulated immune system pathways.

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Exposure to polybrominated biphenyl (PBB) associates with genome-wide DNA methylation differences in peripheral blood.

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Project description:Purpose: To compare RNASeq data of Frankia inefficax EuI1c in normal growth conditions to biphenyl-stressed Frankia inefficax EuI1c. Frankia cultures were grown for 3 days under one of three conditions before harvesting: 1.) 1 mM biphenyl stress with glucose as an alternative carbon and energy source, 2.) 1mM biphenyl with no alternative carbon or energy source, or 3.) media with glucose as a carbon and energy source (Control). RNA-seq analysis provided insight into how the the bacteria responds to biphenyl stress and exhibits biphenyl tolerance.

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Polybrominated diphenyl ethers (PBDEs)

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