Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

10X genomic single cell RNA-seq of WT and p47phox-KI lungs with acute lung injury

ABSTRACT: 10X genomic single cell RNA-seq of WT and p47phox-KI lungs with acute lung injury

PROVIDER: PRJNA554875 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9693901_1.fastq.gz	Fastqsanger.gz
	SRR9693901_2.fastq.gz	Fastqsanger.gz
	SRR9693902_1.fastq.gz	Fastqsanger.gz
	SRR9693902_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

10X genomic single cell RNA-seq of WT and p47phox-KI lungs with acute lung injury

Project description:We performed single cell RNA sequencing of CD45-negative cells sorted from WT and p47phox-KI lungs with ALI to characterize the impacts of the loss of p47phox phosphorylation in blood cells on pulmonary endothelial and epithelial cells. We found many genes are pertinent to enhancement of pulmonary vasculature integrity, maintain alveolar roles, promote ATII cell proliferation and lower oxidative stress state in p47phox-KI lungs.

2020-07-10 | GSE134365 | GEO

RNAseq on lungs from WT vs claudin 4 KO mice that underwent ventilator-induced lung injury

Project description:WT mice and claudin 4 KO mice were exposed to ventilator-induced lung injury (VILI) for 2 hours. We found that in some Cldn4 KO mice, injury was similar to WT, while in others, injury was higher, as assessed by amount of protein leak into broncho-alveolar lavage fluid. We performed RNAseq to find which genes were responsible for higher injury in Cldn4 KO mice. WT mice and claudin 4 KO mice were exposed to ventilator-induced lung injury (VILI) for 2 hours. RNA were extracted from whole lungs and RNA sequencing was performed. The samples are (all in duplicates): WT no VILI, Cldn4 KO no VILI, WT VILI, Cldn4 KO VILI with similar injury to WT (Cldn4 KOlow), and Cldn4 KO VILI with higher injury than WT (Cldn4 KOhigh)

2014-11-04 | E-GEOD-50927 | biostudies-arrayexpress

Gene expression analysis in lung fibroblasts from p38+/+ (wt) and p38ki/ki (ki) mice

Project description:To assess differently expressed genes beween wt and ki fibroblasts Total RNA was purified from fibroblasts of 3 different mice for wt and 3 different mice for ki

2016-06-01 | E-GEOD-80825 | biostudies-arrayexpress

Gene expression analysis in lung fibroblasts from p38+/+ (wt) and p38ki/ki (ki) mice

Project description:To assess differently expressed genes beween wt and ki fibroblasts

2016-06-01 | GSE80825 | GEO

Murine colonic epithelial cells: Trabid(Zranb1) C443S hetero knock-in mouse (KI/+) vs. homo knock-in mouse (KI/KI)

Project description:Transcriptional profiling of colonic epithelial cells comparing control Trabid KI/+ with KI/KI

2023-05-24 | GSE204668 | GEO

Ad-HER-wt and Ad-HER2-ki infected HMECs

Project description:As an oncogene, use of HER2 vaccines in humans requires the development of HER2 immunotherapies with maximal immunologic potential, but minimal oncologic potential. To address these issues, we developed a recombinant adenoviral vector expressing a mutated HER2 inactivated for kinase function (Ad-HER2-ki). Ad-HER2-ki was highly expressed, but non-phosphorylated and elicited minimal transcription dysregulation in primary cells. In contrast, Ad-HER2-wt elicited a strong oncogenic signature associated with tumorigenesis.

2008-11-15 | GSE13274 | GEO

Ki-67 organises heterochromatin during cell proliferation

Project description:Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not inhibit cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 at heterochromatin was strongly reduced. Overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression.

2018-10-26 | PXD003551 | Pride

RNA-seq of Setx KI and WT Spinal Cord

Project description:The goal of this study is to compare differences in splicing between bulk spinal cord samples isolated from Setx KI and WT mice

2022-04-22 | GSE199664 | GEO

Analysis of UTI on WT and PI3KδE1020K-B KI mice

Project description:Either WT or PI3KδE1020K-B KI mice were infected with UPEC trans urethrealy twice 1 hour apart and culled 12 hours later. Kidneys were subsequently perfused, RNA extracted and analysed via RNASeq

2022-11-01 | GSE139849 | GEO

Transcription profiling of rat mechanically ventilated lungs from strains resistant or susceptible to ventilation-associated lung injury (VALI)

Project description:We sought to confirm the genetic influence in the development of Ventilation-Associated Lung Injury (VALI) and, in the process, identify potential candidate genes involved in the disease by integrating differential gene expression profiling on rat lungs to a traditional strain survey analysis of the parental rat strains, VALI-sensitive Brown Norway rats versus VALI-resistant Dahl Salt Sensitive rats, comparing control (under room air ventilation) versus under high tidal volume (HTV) ventilation. We used microarrays to detail the global programme of gene expression underlying VALI susceptibility and identified distinct classes of up-regulated and down-regulated genes during this process. Experiment Overall Design: Rat lungs (Right lungs) from both sets of parents under the two conditions (control vs. HTV ventilation) were isolated for RNA extraction and hybridization on Affymetrix microarrays.

2007-10-31 | E-GEOD-7041 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data