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RNA-Seq analysis of the Neisseria gonorrhoeae GntR-type transcriptional regulator GdhR

ABSTRACT: RNA-Seq analysis of the Neisseria gonorrhoeae GntR-type transcriptional regulator GdhR

PROVIDER: PRJNA556885 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9845620.fastq.gz	Fastqsanger.gz
	SRR9845620_1.fastq.gz	Fastqsanger.gz
	SRR9845620_2.fastq.gz	Fastqsanger.gz
	SRR9845621.fastq.gz	Fastqsanger.gz
	SRR9845621_1.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis of the Neisseria gonorrhoeae GntR-type transcriptional regulator GdhR

Project description:In this work we have determined by RNA-Seq the whole set of genes regulated by the N. gonorrhoeae GdhR transcriptional regulator (NGO1360 gene in reference strain FA1090). The results showed that GdhR regulates the expression of 2.3% of all the genes in the gonococcal (strain FA19) genome, of which 39 were activated and 11 were repressed. Most of the GdhR-regulated genes lie in the category of fimbrial proteins and membrane antigens or transporters. GdhR was found not be a cryptic regulator since there is an overlap in the set of GdhR-regulated genes between the wild type and a strain expressing gdhR ectopically from the lac promoter. Among the GdhR-regulated genes linked to gonococcal pathogenesis we found that lctP, encoding a unique L-lactate transporter, was the highest of GdhR-repressed genes.

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