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TCP5 controls leaf margin development by regulating the KNOX and BEL-like transcription factors in Arabidopsis

ABSTRACT: TCP5 controls leaf margin development by regulating the KNOX and BEL-like transcription factors in Arabidopsis

PROVIDER: PRJNA558361 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9891690_1.fastq.gz	Fastqsanger.gz
	SRR9891690_2.fastq.gz	Fastqsanger.gz
	SRR9891691_1.fastq.gz	Fastqsanger.gz
	SRR9891691_2.fastq.gz	Fastqsanger.gz
	SRR9891692_1.fastq.gz	Fastqsanger.gz

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TCP5 controls leaf margin development by regulating the KNOX and BEL-like transcription factors in Arabidopsis

Project description:In this supplemental high-throughput data, we report TCP5 downstream genes in leaf marginal development by RNAseq, using 12-day old seedlings derived from the inducible over-expression lines (35S:GR-TCP5).

2019-08-03 | GSE135305 | GEO

Effects of corosolic acid on gene expression of in Bel-7402 and Bel-7404 cells

Project description:The purpose of this study was to investigate gene expression in corosolic acid-treated liver cancer cells (Bel-7402 and Bel-7404)

2022-09-15 | GSE201455 | GEO

Next-generation sequencing analysis of Piwi-interacting RNAs (piRNAs) in Drosophila wild-type and bel mutant ovaries

Project description:Belle has been known to be co-localized with piRNA-related proteins at the nuage of germline cells during Drosophila oogenesis. However, its role in piRNA biogenesis remains unclear. To reveal whether Belle is involved in regulating piRNA expression, we performed next-generation sequencing analysis of small non-coding RNAs on ovaries harvested from the wild type (W1118) and trans-heterozygous bel[74407/neo30] mutant. Small RNA-seq experiments were performed on three individual ovary samples with the same genotype. For piRNA expression analysis, we performed mapping of three sets of small RNA sequencing reads for each genotype to previously identified eight distinct piRNA clusters located in four different Drosophila chromosomes (from X to 4). Analysis of the piRNA expression profiling from these piRNA cluster loci indicates that some specific piRNA populations were either upregulated or downregulated in bel mutant ovaries compared with wild-type ovaries. Furthermore, we performed systematic analysis by mapping piRNA sequencing reads to sequences of all identified Drosophila transposable elements (TEs) to classify and measure piRNA reads based on their TE targets. Among 124 TE-classified piRNA populations, 9 and 20 of them were upregulated and downregulated (≥2 folds), respectively, in bel74407/neo30 mutant ovaries compared with those from wild-type ovaries. To examine the effect of the bel[74407/neo30] mutation on the ping-pong cycle for secondary piRNA biogenesis, analysis of the ping-pong signature of piRNAs specifically mapped to the retro-element Burdock was performed. The ping-pong signature for the generation of secondary piRNAs was not significantly altered in bel mutants compared with the wild type. These results, taken together, indicate that Bel is not required for primary and secondary piRNA biogenesis, but it is involved in regulating expression of specific subsets of piRNA populations.

2017-09-01 | GSE65565 | GEO

Achromobacter sp. Bel

Project description:Achromobacter sp. Bel Genome sequencing and assembly

| PRJNA625632 | ENA

Different gene expression between CENPK knockdown and control hepatocellular carcinoma cell line BEL-7404

Project description:We sought to find out the molecular mechanism of CENPK displayed in hepatocellular carcinoma. Microarray experiments were carried out to identify different gene expression between CENPK-knockdown BEL-7404 cell line and Scramble BEL-7404 cell lines.

2018-05-30 | GSE114983 | GEO

iTRAQ experiment to identify proteins that changed before and after NMT1 was knocked down in both Bel-7402 and Bel-7404 cells

Project description:We performed proteomics isobaric tags for relative and absolute quantification (iTraq) to identify proteins that changed before and after NMT1 was knocked down in both Bel-7402 and Bel-7404 cells.

2021-09-08 | PXD011812 | Pride

Expression data of GTSE1-knockdown hepatoma cell line Bel-7404

Project description:Liver cancer is the fourth deadliest cancer worldwide and is in lack of prognostic indicators. As a p53 inducible gene, GTSE1 (G2 and S phase-expressed-1) showed widely correlation with tumor progress and prognosis in clinical, while its potency as a prognosis indicator or molecular mechanism in regulating cell cycle remain little known. This study aimed to elucidate clinical values and potential functions of GTSE1 in liver cancer. In study of GTSE1 functions, Bel-7404 cell, a human hepatoma cell line, was induced GTSE1-knockdown using lentiviral-mediated RNAi and performed RNA microarray analysis.

2019-04-20 | GSE130083 | GEO

microRNA expression of TRAIL-sensitive and TRAIL-resistant Bel-7402 cells

Project description:To further analyze the change of microRNA (miRNA) between TRAIL-sensitive and TRAIL-resistant Bel-7402 cells Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)

2015-10-20 | GSE74130 | GEO

CINCINNATA Controls Surface Curvature of Antirrhinum Leaf by Coordinated and Direct Activation of Cytokinin and Auxin Signaling

Project description:Mutations in the CINCINNATA gene in Antirrhinum and its orthologues in Arabidopsis cause negative surface curvature in leaves due to excess marginal growth. CIN-like genes code for TCP transcription factors and are expressed in a broad zone of a growing leaf somewhat distal to the proliferation zone. Although a few TCP targets are known, the role of CIN-like TCP genes in regulating leaf curvature has remained unclear. We have compared the global transcription profile of wild type and cincinnata mutant to identify its targets. By combining DNA-protein interaction, chromatin immunoprecipitation and RNA in situ hybridization, we show that CIN maintains surface flatness by regulating signaling or level of major plant hormones. CIN promotes cytokinin signaling directly and GA level indirectly, in accelerating maturity in leaf cells along the tip-to-base direction. In addition, CIN suppresses auxin signaling more at the margin than centre by establishing a margin-to-medial expression gradient of a homologue of the auxin suppressor IAA3. Our results uncover an underlying mechanism in a developing leaf that controls maturity of leaf and its surface curvature. Considering the conservation of CIN-like genes and their function in leaf morphogenesis in multiple plant species, it is likely that such mechanism is evolutionarily conserved.

2013-05-01 | GSE40621 | GEO

Effects of corosolic acid on gene expression of in Bel-7402 and Bel-7404 cells

Project description:Effects of corosolic acid on gene expression of in Bel-7402 and Bel-7404 cells

| PRJNA831821 | ENA