Project description:Sampada and Sujata are two contrasting genotypes of Papaver somniferum that are contrasting in terms of their latex and alkaloid profiles. The major objective of the present study was to use a small-scale (750 target genes) microarray of P. somniferum for identification of genes that are differentially expressed in the capsule walls of the two contrasting genotypes, Sampada and Sujata. Nidarshana Chaturvedi and Mridula Singh made equal contribution as first authors to this data.
Project description:Sustainable production of switchgrass (Panicum virgatum) as a bioenergy crop hinges in part on efficient use of soil macronutrients, especially nitrogen (N). This study investigated the physiological, metabolic and transcriptomic responses of switchgrass to N limitation. Moderate N limitation marked a tipping point for large changes in plant growth, root-to-shoot ratio, root system architecture and total nitrogen content. Integration of transcriptomic and metabolic data revealed that N limitation reduced switchgrass photosynthetic capacity and carbon(C)-fixation activities. Switchgrass balanced C-fixation with N-assimilation, transport and recycling of N compounds by rerouting C-flux from glycolysis, the oxidative branch of the pentose phosphate pathway (OPPP) and from the tricarboxylic acid (TCA) cycle in an organ specific manner. The energy and reduction power so generated, and C-skeletons appear to be directed towards N uptake, biosynthesis of energy storage compounds with high C/N ratio such as sucrose, non-N-containing lipids, and various branches of secondary metabolism.
Project description:affy_floralangers_rose - affy_floral_rose - - Which genes are induced during floral initiation? - Are the genes involved in floral initiation identical between our 3 genotypes? - Which genes are involved in the control of recurrent blooming in rose? - Which genes are diferentially expressed between buds that will become floral and buds that will remain vegetative?-This project aims to find in rose genes involved in flowering control (floral initiation and recurrent flowering). First, the floral initiation will be observed in 3 genotypes. Then we will check if same genes are regulated within genotypes for this process. Concerning recurrent blooming, we will compare flower bud versus vegetative buds in non-recurrent conditions and finally bud from non-recurrent and recurrent genotypes. Keywords: time course
Project description:In light of the changes in precipitation and soil water availability expected with climate change, understanding the mechanisms underlying plant responses to water deficit is essential. Toward that end we have conducted an integrative analysis of responses to drought stress in the perennial C4 grass and biofuel crop, Panicum virgatum (switchgrass). Responses to soil drying and re-watering were measured at transcriptional, physiological, and metabolomic levels. To assess the interaction of soil moisture with diel light:dark cycles, we profiled gene expression in drought and control treatments under pre-dawn and mid-day conditions. Soil drying resulted in reduced leaf water potential, gas exchange, and chlorophyll fluorescence along with differential expression of a large fraction of the transcriptome (37%). Many transcripts responded differently depending on time of day (e.g. up-regulation pre-dawn and down-regulation mid-day). Genes associated with C4 photosynthesis were down-regulated during drought, while C4 metabolic intermediates accumulated. Rapid changes in gene expression were observed during recovery from drought, along with increased water use efficiency and chlorophyll fluorescence. Our findings demonstrate that drought responsive gene expression depends strongly on time of day and that gene expression is extensively modified during the first few hours of drought recovery. Analysis of covariation in gene expression, metabolite abundance, and physiology among plants revealed non-linear relationships that suggest critical thresholds in drought stress responses. Future studies may benefit from evaluating these thresholds among diverse accessions of switchgrass and other C4 grasses.
Project description:Cereal aphids can successfully colonize and damage switchgrass (Panicum virgatum) plants. Among the aphids tested, greenbugs (Schizaphis graminum, GB) caused significant plant damage likely through a combination of aphid-salivary proteins that are injected into plants during feeding and a strong host response elicited by herbivory. In this study, shotgun label-free proteomics has been used to document changes to the switchgrass proteome as a result of GB infestation. These proteomic data were compared against transcriptome changes recently published for this system.
2022-02-04 | PXD018547 | Pride
Project description:Transcriptome Analysis Reveals Genes Differentially Expressed in Muscle
Project description:We used a digital gene expression (DGE) system to generate partial gene expression profiles for 12 selected samples. Significant differences in gene expression between early- and late-flowering samples were detected for 72 candidate genes for flowering time. Genes related to circadian rhythms were significantly overrepresented among the differentially expressed genes. Our data suggest that circadian clock genes play an important role in the evolution of flowering time, and C. bursa-pastoris plants exhibit expression differences for candidate genes likely to affect flowering time across the broad range of environments they face in China.
Project description:This is a comparative experiments of three barley genotypes harbouring allelic differences at a locus designated QRMC-3HS putatively implicated in the assembly of the microbial communities thriving at the root-soil interface, the so called rhizosphere microbiota. The RNA-seq experiment aimed at identify genes differentially regulated among the genotypes at the locus of interest. As the selected genotypes host contrasting microbiotas, we hypothesised that differentially expressed genes at the locus represent primary candidates for the trait of interest (i.e., microbiota recruitment).