MiRNAs in the porcine corpus luteum
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ABSTRACT:
PROVIDER: PRJNA591167 | ENA |
REPOSITORIES: ENA
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Project description:Recently, microRNAs (miRNAs) have emerged as new players in the fine tuning of some reproductive functions in mammals via posttranscriptional gene regulation mechanisms. Importantly, miRNAs have been suggested to be an important regulators of various ovarian functions. Applying custom made multispecies arrays we aimed to analyze expression profile of miRNAs in corpus luteum to answer the question whether miRNAs can be involved in maintenance of luteal function during early pregnancy in pigs.
2020-04-21 | GSE140854 | GEO
Project description:The corpus luteum plays a critical role in reproduction because it is the primary source of circulating progesterone. This study aimed to determine the in vitro effect of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the transcriptome genes expression in the porcine corpus luteum in the mid- and late-luteal phase of the estrous cycle using RNA-seq technology. The corpus luteum slices were incubated in vitro in the presence of PPARγ agonist – pioglitazone and antagonist—T0070907. We identified 40 differentially expressed genes after pioglitazone treatment and 40 after T0070907 treatment in the mid-luteal phase as well as 26 after pioglitazone and 29 after T0070907 treatment in late-luteal phase of the estrous cycle. In addition, we detected differences in genes expression between the mid- and late-luteal phase without treatment (409). These results should become a basis for further studies explaining the mechanism of PPARγ action in the reproductive system in pigs.
2022-11-15 | E-MTAB-11964 | biostudies-arrayexpress
Project description:Transcriptomic profile of porcine corpus luteum after PPAR gamma ligands treatment
| PRJEB54775 | ENA
Project description:Observational data suggest that women conceiving without a corpus luteum are at higher risk of developing preeclampsia. While the underlying mechanisms remain unclear, the absence of corpus luteum-derived secretory products may be a contributing factor. This study investigates whether the plasma proteome differs between women who conceive with or without a corpus luteum and examines the relationship with mode of conception. Plasma samples from 12 participants were collected at three time points: first trimester, third trimester, and postpartum. The cohort included women who conceived unassisted (UC) after infertility, via artificial cycle frozen embryo transfer (AC FET), or natural cycle frozen embryo transfer (NC FET). 36 plasma protein samples were analyzed using mass spectrometry-based proteomics to compare the proteome of women who conceived with and without a corpus luteum, across different conception methods and pregnancy stages. In total, 528 proteins were quantified. No differentially expressed plasma proteins were identified between women with and without a corpus luteum. However, 15 proteins showed differential expression between UC and FET at all time points, with Bonferroni-corrected p<9.47 × 10E-5 and FC≥|2|. Several altered proteins, including PAPPA and ANG, were linked to preeclampsia. SERPINA7 was differentially detected when comparing time points within the unassisted conception method. No significant differences were detected between AC FET and NC FET. This pilot study revealed a unique proteomic signature associated with mode of conception. The findings suggest biologically plausible candidate proteins for further testing. Validation in larger cohorts or with alternative proteome analysis technologies is needed
2025-09-30 | PXD067974 | Pride
Project description:Effect of LPS on corpus luteum - TMT-based approach
2024-03-28 | MSV000094426 | MassIVE
Project description:To investigate the rapid effects of PGF2α on the corpus luteum we performed gene expression profiling analysis using data obtained from RNA-seq of controls and treatments at two time points with 4 biological replicates/group .
2022-11-04 | GSE217053 | GEO
Project description:In order to indentify genes regulated by eCG, and involved in CL development and progesterone increases, the transcriptome was evaluated using the microarray technology Cows (Bos indicus) were divided into control, stimulated and superovulated groups. The stimulated group received 400 IU of eCG on day 8 and the superovulated group received 2000 IU of eCG on day 4 after the beginning of synchronization. Corpus luteum were collected at day 6 after ovulation e the trasncripitome was available by microarray.
2012-05-08 | E-GEOD-37844 | biostudies-arrayexpress
Project description:Temporal changes in the corpus luteum during early pregnancy
| PRJNA615852 | ENA
Project description:Although rescue of the corpus luteum is required for pregnancy, luteal function during maternal recognition of pregnancy remains largely unexplored. CL were collected from pregnant cattle on days 14, 17, 20, and 23, to encompass the maternal recognition of pregnancy period. Nanostring technology was used to profile miRNA. A total of 27 miRNA changed. MiRNA that increased were predicted to inhibit phosphatidylinositol signaling, while those that decreased may be negative regulators of steroidogenesis. Overall, these data indicate that there are changes in the CL of pregnancy that are important for continued luteal function.
2020-03-28 | GSE147623 | GEO
Project description:Lactation and associated metabolic stresses during the post-partum period have been shown to impair fertility in dairy cows. The oviduct plays key roles in embryo development and the establishment of pregnancy in cattle. The aim of this study was to investigate the effects of lactation and location relative to the corpus luteum (CL) on the transcriptome of the bovine oviduct epithelium.
2019-01-07 | GSE124110 | GEO