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Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Brd4

ABSTRACT: Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Brd4

PROVIDER: PRJNA594708 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10640875.fastq.gz	Fastqsanger.gz
	SRR10640876.fastq.gz	Fastqsanger.gz
	SRR10640877.fastq.gz	Fastqsanger.gz
	SRR10640878.fastq.gz	Fastqsanger.gz
	SRR10640879.fastq.gz	Fastqsanger.gz

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Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Brd4

Project description:We transfected C2C12 cells with 100nM of scrambled siRNA scramble and an siRNA directed against Brd4. Cells were harvested at 48h post-transfection and RNAseq was performed with polyA selection

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Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Fndc1

Project description:We transfected C2C12 cells with 100nM of scrambled siRNA scramble and an siRNA directed against Fndc1. Cells were harvested at 48h post-transfection and RNAseq was performed with polyA selection

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Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Fndc1

Project description:Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Fndc1

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Effect of H19 Knockdown by siRNA on gene expression in C2C12 cells

Project description:We used microarrays to characterize the global changes in gene expression in C2C12 cells due to siRNA knockdown of long non-coding RNA H19

2013-09-30 | GSE49535 | GEO

Effect of H19 Knockdown by siRNA on gene expression in C2C12 cells

Project description:We used microarrays to characterize the global changes in gene expression in C2C12 cells due to siRNA knockdown of long non-coding RNA H19 Control siRNA or siRNA specific for mouse H19 were transfected into day1 differentiating C2C12 myoblasts in triplicates. 40 H later total RNAs were isolated and subjected with microarray analysis.

2013-09-30 | E-GEOD-49535 | biostudies-arrayexpress

PRC SiRNA vs scramble transfection in XTC.UC1 cells

Project description:PGC-1 related coactivator (PRC) shares structural and functional features with PGC-1{alpha}. It regulates several metabolic pathways and mitochondrial biogenesis. Its specific role in the early programming of cell proliferation suggests a finely regulated crosstalk between the mitochondrial functions and the cell cycle status. To explore the PRC-regulated pathways, we used a cell-line model of mitochondrial-rich tumors, presenting an oxidative metabolism and a specific increase in PRC expression. Microarray studies of temporal PRC invalidation in these XTC.UC1 cells were compared to the functional status of the mitochondria as well as to the expression level of genes and proteins involved in the oxidative phosphorylation process. Compared with what was observed for PGC-1{alpha}, we explored the role of nitric oxide in the PRC-regulated mitochondrial biogenesis. We proved that nitric oxide rapidly influences the expression of PRC at the transcriptional level. Focusing on mitochondrial energy metabolism, we demonstrated that PRC differentially controls the respiratory chain complexes and the coupling efficiency, in order to conserve a sufficient level of ATP and to protect the cell from oxidative stress. Our results highlight the key role of the PRC coactivator in the fine modulation of metabolic functions in response to the cell cycle status. Keywords: Transcriptionnal coactivator invalidation by SiRNA Expression profiles at 0h, 12h, 24h and 48h of PRC SIRNA treatment compared to scramble and referred to time of 20% serum induction. Expression profiles of PRC SIRNA and scramble during serum starvation were referred as -1.

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siRNA profiling of mouse myoblast C2C12 cells reveals PPARbeta/delta dependent differentiation

Project description:siRNA profiling of mouse myoblast C2C12 cells reveals PPARbeta/delta dependent differentiation

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Transcriptomic analysis after transfection in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) of either a siRNA against SOX2 or a scramble siRNA

Project description:SOX2 is the main gene involved in anophthalmia. In order to identify genes regulated by SOX2 transcription factors (genes that could be good candidates to also be involved in ocular development), we studied transcriptomic profiles of murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) after transfection by a siRNA against SOX2 or a scramble siRNA. murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) after transfection by a siRNA against SOX2 or a scramble siRNA

2015-11-04 | E-GEOD-74598 | biostudies-arrayexpress

Transcriptomic analysis after transfection in murine genetically modified stem cells overexpressing the RAX gene (CCE-Rx cells) of either a siRNA against SOX2 or a scramble siRNA

2015-11-04 | GSE74598 | GEO

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2022-10-01 | GSE186225 | GEO

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