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MicroRNA profiling in different types,grades and stages of breast cancer

ABSTRACT: MicroRNA profiling in different types,grades and stages of breast cancer

PROVIDER: PRJNA603741 | ENA |

REPOSITORIES: ENA

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MicroRNA profiling in different types,grades and stages of breast cancer

Project description:MicroRNA profiling was done using Taqman Low Density Arrays (TLDA) platform consisting of 667 microRNAs covering Sanger miRBase version10 cross 50 samples along with their adjacent normals consisting of different types (ER+ and ER-ve), grades (grade 2 and grade 3) with their different stages (I to III). The above isolated RNA which displayed good RIN value and linearity (R2>0.96), were used for reverse transcription (RT) reactions with the help of TaqMan MicroRNA Reverse Transcription Kit followed by real time PCR Reactions (ABI 7900 HT) as per manufacturer's instructions.A pre -amplification step of cDNA with pre-amp megaplex pool primers was carried out to significantly enhance the ability to detect highly down regulated miRNAs. The TaqMan human microRNA arrays consists of two plates, pool A and pool B. RNU 46 and RNU 48 were used as endogenous controls for data normalization. Another control not related to human was also included as a negative control. Each TaqMan Assay was run in quadruplicate. RNU 46 and RNU 48 expression was consistent in all the samples and displayed good range of CT values (22–24 ) where as in ‘No Template’ Control (NTC) CT value was above 38. The average CT values of total profiled miRNAs in all samples were normalized with RNU 46 & RNU 48 using spotfire (statminer) software and fold changes were represented in terms of 2 - Δ Δ CT (RQ ) and log10RQ. Only valid and significant miRNAs were picked up for further analysis.

2020-01-30 | GSE144463 | GEO

MicroRNA signatures differentiate types, grades, and stages of breast invasive ductal carcinoma (IDC): miRNA-target interacting signaling pathways.

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microRNA profiling in different stages of rheumatoid arthritis

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Transcriptional profiling of ovarian cancer samples with different grades

Project description:We have previously demonstrated (Calura E et al., 2013) that different epithelial ovarian cancer (EOC) histotypes have different, defined characteristics at the microRNA level, which might highlight a different originating tissue for the disease. From these results we have invesigated whole-genome gene expression on a cohort of samples with different FIGO grades, in order to investigate the link between the tumor grade and gene expression.

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Miniature pigs tooth germ tissue: four different types of teeth in three different developing stages

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MiRNA profiling of meningioma of different grades and histological subtypes

Project description:Although meningiomas are one of the most frequent primary intracranial tumors, there are only a few studies dealing with gene regulation processes in meningiomas. MiRNAs are key regulators of gene expression and regulation and miRNA profiles offer themselves as biomarkers for cancer development and progression. To investigate the role of miRNAs during meningioma growth and progression, we compared expression of 1205 miRNAs in 55 meningioma samples of different tumor grades and histological subtypes. We were able to classify histological subtypes in WHO grade I meningiomas with up to 97% accuracy (meningothelial versus fibroblastic) and different WHO grades with up to 93% accuracy (WHO I versus WHO III). We found significant downregulation of miRNAs on chromosome 1p36 and within two large miRNA clusters on 14q32 in high grade meningiomas, two regions that are yet associated with meningioma progression. We also identified several miRNAs associated with epithelial to mesenchymal transition differentially expressed in meningothelial meningioma compared to fibroblastic meningioma. Combined, our data show that meningiomas of different WHO grades and histological subtypes show a specific miRNA expression profile. Some individual miRNAs can also serve as potential biomarkers for meningioma progression.

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Expression data from different stages and types of oocytes and cumulus cells (CCs)

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2010-03-23 | E-GEOD-21005 | biostudies-arrayexpress

Expression data from different stages and types of oocytes and cumulus cells (CCs)

2010-03-23 | GSE21005 | GEO

Miniature pigs tooth germ tissue: four different types of teeth in three different developing stages

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