Project description:The study aimed to characterize plasmids mediating carbepenem resistance in Klebsiella pneumoniae in Pretoria, South Africa. We analysed 56 K. pneumoniae isolates collected from academic hospital around Pretoria. Based on phenotypic and molecular results of these isolates, 6 representative isolates were chosen for further analysis using long reads sequencing platform. We observed multidrug resistant phenotype in all these isolates, including resistance to aminoglycosides, tetracycline, phenicol, fosfomycin, floroquinolones, and beta-lactams antibiotics. The blaOXA-48/181 and blaNDM-1/7 were manily the plasmid-mediated carbapenemases responsible for carbapenem resistance in the K. pneumoniae isolates in these academic hospitals. These carbapenemase genes were mainly associated with plasmid replicon groups IncF, IncL/M, IncA/C, and IncX3. This study showed plasmid-mediated carbapenemase spread of blaOXA and blaNDM genes mediated by conjugative plasmids in Pretoria hospitals.
Project description:We use the bacterium Klebsiella spp. as a model organism, we are interested in the bacterial capsule and the functional and evolutionnary consequences of capsule exchanges.
2026-02-03 | GSE306874 | GEO
Project description:Genomic characterization of isolates from hospital effluents in KZN, South Africa
Project description:This data was generated by the CHAIN cohort was primary aim was to identify the biological mechanisms underlying mortality among young children in sub-Saharan Africa and South Asia. Acutely ill children (n=3101) were admitted into the study at hospital admission. Children were then followed up for 6 months after hospital admission at days 45, 90 and 180. Blood was collected at hospital admission, discharge from hospital and follow-up time points. Plasma proteins were quantified from the blood collected at admission and discharge points.