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ABSTRACT: Tropical pitcher plants (Nepenthes) act as ecological filters by altering properties of their fluid microenvironments

PROVIDER: PRJNA605027 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11029800.fastq.gz	Fastqsanger.gz
	SRR11029801.fastq.gz	Fastqsanger.gz
	SRR11029802.fastq.gz	Fastqsanger.gz
	SRR11029803.fastq.gz	Fastqsanger.gz
	SRR11029804.fastq.gz	Fastqsanger.gz

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Pitcher fluid protein profiling of Nepenthes x ventrata

Project description:We studied protein profile in the first three days upon pitcher opening to understand carnivory trait of Nepenthes x ventrata. The proteome analysis of pitcher fluid from N. × ventrata was performed by mass spectrometry (nLC-MS/MSALL).

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Pitcher fluid protein profiling of Nepenthes ampullaria

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Mass spectrometry data of metabolomics analysis of Nepenthes pitchers

Project description:Hybridisation plays a significant role in the evolution and diversification of plants. Hybridisation among Nepenthes species is extensive, either naturally or man-made. To investigate the effects of hybridisation on the chemical compositions, we carried out metabolomics study on pitcher tissue of Nepenthes ampullaria, Nepenthes rafflesiana and their hybrid, Nepenthes × hookeriana. Pitcher samples were harvested and extracted in methanol:chloroform:water via sonication-assisted extraction before analysed using LC-TOF-MS. MS data were analysed using XCMS online version 2.2.5. This is the first MS data report towards the profiling, identification and comprehensive comparison of metabolites present in Nepenthes species.

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Project description:Plants from the Nepenthes genus, which enumerates approximately 120 species, possess specialized pitchers enabling them to capture and digest various preys, mainly arthropods, from which the plants derive nutrients. The pitcher fluid contains many molecules of noteworthy importance, including antimicrobial compounds, traditionally used in medicine, as well as hydrolytic enzymes for prey digestion. In this study, polyesters films made from poly(ethylene terephthalate) (PET) and from poly(butylene adipate -co- terephthalate) (PBAT) were incubated in the pitcher of the carnivorous plants Nepenthes alata and Sarracenia purpurea. High performance liquid chromatography analysis revealed hydrolysis into their corresponding monomers, while hydrolysis efficiency was up to ten times higher in the presence of dried mealworm Tenebrio molitor and jasmonic acid. Proteomic analysis indicated the presence of the aspartic proteinase nepenthesin in most conditions, with high abundance in 70% of polyester-containing conditions compared to those without polyesters. Molecular docking simulations further suggested that nepenthesin has the potential to hydrolyze polyesters. While in contrast to cutinases there is only little information on hydrolysis of PET and PBAT by proteases in the literature, this work clearly demonstrates hydrolysis of both PET and PBAT by the recombinant protease nepenthesin, releasing similar amounts of TPA as the cutinase from Humicola insolens. These results suggest carnivorous plant fluids as a source for new enzymes for industrial applications such as for polyester hydrolysis.

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