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Genome wide expression analysis of senescent BJ fibroblasts in the presence or absence of various SMAD2/3 regulating molecules

ABSTRACT: Genome wide expression analysis of senescent BJ fibroblasts in the presence or absence of various SMAD2/3 regulating molecules

PROVIDER: PRJNA610885 | ENA |

REPOSITORIES: ENA

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Genome wide expression analysis of senescent BJ fibroblasts in the presence or absence of various SMAD2/3 regulating molecules

Project description:BJ fibroblasts were grown to replicative senescence, and were then treated with control or SMAD2/3 regulating ligands. RNA was then harvested for RNA-seq.

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Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance, in the presence or absence of various SMAD2/3 regulating molecules

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Genome wide mapping of NFI and SMAD2/3 binding in senescence

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Genome wide expression analysis of young vs senescent BJ fibroblasts

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miRNA Expression in Senescent and Immortalized BJ fibroblasts

Project description:Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs. We used miRNA microarrays to provide a detailed account of miRNA profiles for early passage and senescent human foreskin (BJ) fibroblasts as well as early and late passage immortalized fibroblasts (BJ-hTERT) that stably express the human telomerase reverse transcriptase subunit hTERT. The revelation that miRNA expression changes with extended passaging in BJ-hTERT cells will contribute to a comprehensive understanding of the connections between telomerase expression, senescence and processes of cellular aging.

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Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance, in the presence or absence of various SMAD2/3 regulating molecules

Project description:Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance, in the presence or absence of various SMAD2/3 regulating molecules

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Genome-wide expression analysis of young, senescent and p38MAPK-inhibitited senescent human fibroblasts.

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