Project description:BJ fibroblasts were grown to replicative senescence, and were then treated with control or SMAD2/3 regulating ligands. RNA was then harvested for RNA-seq.
Project description:BJ fibroblasts were grown in the presence of rapamycin until reaching growth arrest at high passages, at which point cells were maintained in or withdrawn from rapamycin, while also being treated with control or SMAD2/3 regulating ligands. RNA was then harvested for RNA-seq
Project description:Quantitative analysis of global proteomic changes in proliferating and senescent BJ fibroblasts, treated with eIF5A hypusination inhibitor GC7 (10 uM) for 12h.
Project description:Young-quiescent and senescent BJ fibroblasts were harvested for RNA-seq analysis in the presence or absence of doxycycline treatment.
Project description:Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs. We used miRNA microarrays to provide a detailed account of miRNA profiles for early passage and senescent human foreskin (BJ) fibroblasts as well as early and late passage immortalized fibroblasts (BJ-hTERT) that stably express the human telomerase reverse transcriptase subunit hTERT. The revelation that miRNA expression changes with extended passaging in BJ-hTERT cells will contribute to a comprehensive understanding of the connections between telomerase expression, senescence and processes of cellular aging.
Project description:Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance, in the presence or absence of various SMAD2/3 regulating molecules
Project description:We utilized whole genome sequencing of mRNA (RNA-seq) to understand the extent to which the senescence-associated secretory phenotype is regulated by p38MAPK Examination of replicates of young, senescent or p38MAPK-inhibited senescent BJ human foreskin fibroblasts.