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Single Cell RNA-sequencing of murine back skin interfollicular epidermis basal cells

ABSTRACT: Single Cell RNA-sequencing of murine back skin interfollicular epidermis basal cells

PROVIDER: PRJNA611558 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11271748.fastq.gz	Fastqsanger.gz
	SRR11271748_1.fastq.gz	Fastqsanger.gz
	SRR11271748_2.fastq.gz	Fastqsanger.gz
	SRR11271749_1.fastq.gz	Fastqsanger.gz
	SRR11271749_2.fastq.gz	Fastqsanger.gz

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Single Cell RNA-sequencing of murine back skin interfollicular epidermis basal cells

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Single Cell RNA-sequencing of murine tail interfollicular epidermis basal cells at postnatal days 7, 30 and in adult

Project description:The purpose of this study was to define, at the single cell level, the transcriptionnal profile of murine tail epidermal basal cells during potsnatal growth and to compare with adult homeostatic basal cells. The analysis was performed on Lgr5DTR-EGFP mice (Tian et al., 2011)(knockin mice containing an Enhanced Green Fluorescent Protein (EGFP) under the control of the Lgr5 regulatory region), allowing to identify and exclude Lgr5-expressing cells of the bulge and basal cells of the interfollicular epidermis were enriched using EGFP negative, CD34 negative, alpha6 integrin positive gating. Our single-cell RNA sequencing revealed that epidermal basal cells at P7 form a more uniform population compared to adult basal cells and that heterogeneity starts to appear around P30.

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Project description:The mammalian skin is a crucial barrier, with the epidermis as its protective, renewing outer layer. Loss of epidermal function is lethal, so multiple stem cell populations likely support the interfollicular epidermis (IFE) for survival. We show that KROX20 marks a diverse stem cell population in the upper and middle mouse hair follicle (HF), partially overlapping with known HF stem cells. Lineage tracing reveals that Krox20-lineage cells migrate from the HF to the IFE, contributing to both basal and suprabasal layers. Spatial transcriptomics analysis of the skin was used to confirm this migration.

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Hair Follicle Epithelial Stem Cells Contributing to Interfollicular Epidermis during Homeostasis [single-cell RNA-seq]

Project description:The mammalian skin is a crucial barrier, with the epidermis as its protective, renewing outer layer. Loss of epidermal function is lethal, so multiple stem cell populations likely support the interfollicular epidermis (IFE) for survival. Using single-cell RNA sequencing, we show that KROX20 marks a diverse stem cell population in the upper and middle mouse hair follicle (HF), partially overlapping with known HF stem cells. Lineage tracing reveals that Krox20-lineage cells migrate from the HF to the IFE, contributing to both basal and suprabasal layers.

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