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Trissolcus basalis

ABSTRACT: RNA-seq of Trissolcus basalis

PROVIDER: PRJNA625932 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11563388_1.fastq.gz	Fastqsanger.gz
	SRR11563388_2.fastq.gz	Fastqsanger.gz
	SRR11563389_1.fastq.gz	Fastqsanger.gz
	SRR11563389_2.fastq.gz	Fastqsanger.gz
	SRR11563390_1.fastq.gz	Fastqsanger.gz

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Nucleus basalis of meynert proteome

Project description:In order to analyse the Nucleus Basalis of Meynert proteome in a large-scale format, we used a multi-dimensional fractionation approach which combines isolation of anatomically-defined nuclei, and protein/peptide chromatographic fractionation strategies coupled to mass spectrometry.

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Differential expression of Wnt signalling molecules between pre- and post-menopausal endometrial epithelial cells suggests that a population of putative epithelial stem/progenitor cells reside in the basalis layer

2012-04-11 | GSE35221 | GEO

Project description:The human endometrium is a highly regenerative tissue that undergoes cyclical proliferation, differentiation and shedding each month. The upper functionalis layer of the endometrium is shed in response to circulating levels of estrogen and progesterone, while the lower basalis layer remains. Clonogenic epithelial stem/progenitor cells likely responsible for regenerating endometrial epithelium have been identified in pre-menopausal (Pre-M) and post-menopausal (Post-M) endometrium and may reside in the basalis layer. We undertook a transcriptional profiling of purified epithelial cells from full-thickness Pre-M and Post-M endometrium to identify differentially expressed genes. The hypothesis tested in the present study was that Post-M endometrial epithelial gene profile would be similar to the quiescent basalis epithelium of Pre-M endometrium. We found striking differential gene expression of many Wnt family members between Pre-M and Post-M and other stem cell network genes. Comparative analysis of our endometrial epithelial gene expression profiles to that of endometrial epithelial cells in remodelling endometrium also provides new evidence showing that Post-M endometrial epithelium has a similar gene signature to that of basalis epithelium of menstrual endometrium. Human endometrial tissue was obtained from 8 pre-menopausal and 3 post-menopausal women undergoing hysterectomy for various benign gynaecologic conditions. Endometrial tissue was digested and isolated using combination of DNase and collagenase. Anti-human EpCAM antibody-coated magnetic Dynabeads was used to positively select total epithelial cells from the digested single cell suspensions. Total RNA was extracted from the purified endometrial epithelial cells and hybridised to Illumina Sentrix HT12 beadchip. Resulting data was compared between pre-menopausal and post-menopausal samples.

2012-04-10 | E-GEOD-35221 | biostudies-arrayexpress

First record of <i>Trissolcus basalis</i> (Hymenoptera: Scelionidae) parasitizing <i>Halyomorpha halys</i> (Hemiptera: Pentatomidae) in the United States.

Project description:BackgroundA parasitoid wasp, Trissolcus basalis (Wollaston), was recorded parasitizing eggs of the invasive stink bug Halyomorpha halys (Stål) in the United States. This is the first record of this species parasitizing fresh and frozen eggs of H. halys in the United States.New informationFirst record of Trissolcus basalis parasitizing Halyomorpha halys eggs in the United States.

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Transcription profiling of decidua basalis from women with normal pregnancies or preeclampsia and/or fetal growth restriction

Project description:The aim of the study was to compare gene expression profiles in decidua basalis from cases with complicated pregnancies to those from healthy pregnant controls. Cases included pregnant women with preeclampsia (PE) and/or fetal growth restriction (FGR). Decidual tissue was obtained by vacuum suction of the placental bed after the placenta was delivered. Women with PE and/or FGR were included as cases. PE was defined as persistent hypertension (blood pressure (BP) ¡Ý 140/90 mm Hg), plus proteinuria (¡Ý0.3g/24 h or ¡Ý2+ according to a dipstick test), developing after 20 weeks of pregnancy. FGR implied birth weight under 2 standard deviations (SD) below the expected birth weight, as related to gestational age (GA) and sex. Due to tissue sampling procedures, only cases delivered by cesarean section (CS) were included. Healthy women with normal pregnancies, undergoing CS for various reasons considered irrelevant to the aim of this study (e. g. breech presentation and previous CS), served as controls. Tissue was immediately submerged in a RNA stabilisation solution (RNAlater, Ambion, Huntingdon, U.K.), incubated at 4¡ãC overnight and stored at -80¡ãC

2007-08-31 | E-MEXP-1050 | biostudies-arrayexpress

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