Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-Cell Transcriptome Analysis of Rb1 Conditional Knockout Prospermatogonia

ABSTRACT: Single-Cell Transcriptome Analysis of Rb1 Conditional Knockout Prospermatogonia

PROVIDER: PRJNA627691 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11599831.fastq.gz	Fastqsanger.gz
	SRR11599832.fastq.gz	Fastqsanger.gz
	SRR11599833.fastq.gz	Fastqsanger.gz
	SRR11599834.fastq.gz	Fastqsanger.gz
	SRR11599835.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Single-Cell Transcriptome Analysis of Rb1 Conditional Knockout Prospermatogonia

Project description:Prospermatogonia, precursors to the mature male germline, undergo a period of sustained quiescence during late fetal and early postnatal development. The purpose of this mitotic arrest is not fully understood. Therefore, utilizing conditional ablation of retinoblastoma protein 1 (Rb1) in mice which blocks entry into quiescence, we analyzed prospermatogonia at the cusp of mitotic arrest at embryonic day 14.5. Prospermatogonia from heterozygous controls or conditional knockout males were isolated via fluorescence-activated cell sorting and profiled via single-cell RNA-sequencing (scRNA-seq). Outcomes of scRNA-seq analysis revealed that a metabolic shift to oxidative phosphorylation and meiotic inhibition are disrupted in prospermatogonia that fail to initiate quiescence. Collectively, these findings and others suggest that biosensing mechanisms eliminate prospermatogonia that fail to mitotically arrest and that key layers of programming are applied during quiescence to ensure proper fate specification.

2021-04-27 | GSE149223 | GEO

Conditional DICER1 knockout hESCs

Project description:Conditional DICER1 knockout hESCs

| PRJNA450977 | ENA

Transcriptome profiling of A549 RB1 wild-type/knockout cell lines

Project description:A549 cells were co-transfected with CRISPR/Cas9 (containing sgRNA and GFP) and HDR donor plasmids (containing RFP and puromycin). The genetic editing cells were selected by puromycin. And then, we performed RNA sequencing for transcription profiling of A549 RB1 RB1 wild-type/knockout cell lines. The raw data were processed with Tophat and cuffdiff for gene expression analysis.

2020-08-25 | GSE132722 | GEO

RNA sequencing of wildtype, PTEN single knockout (SKO), and PTEN;Rb1 double knockout (DKO) mouse prostate organoids

Project description:The goal of this study was to perform transcriptomics on wildtype, PTEN single knockout (SKO) and PTEN;Rb1 double knockout (DKO) mouse prostate organoids. We isolated basal cells from PTEN floxed and PTEN;Rb1 floxed mouse prostates and infected with either RFP control or Cre recombinase to establish wildtype, SKO, and DKO mouse prostate organoids.

2023-09-01 | GSE221020 | GEO

DYRK1A Inhibition via Conditional Knockout

Project description:DYRK1A Inhibition via Conditional Knockout

| PRJNA1171843 | ENA

Conditional Dmrt1 knockout gene expression

Project description:This SuperSeries is composed of the following subset Series: GSE22500: Conditional Dmrt1 knockout gene expression (phase 6) GSE22507: Conditional Dmrt1 knockout gene expression (phase 7) GSE22508: Conditional Dmrt1 knockout gene expression (phase 8) Refer to individual Series

2010-06-30 | E-GEOD-22510 | biostudies-arrayexpress

Transcriptome profiling of A549 RB1 wild-type/knockout cell lines

Project description:Transcriptome profiling of A549 RB1 wild-type/knockout cell lines

| PRJNA548808 | ENA

The impact of conditional Opa1 Knockout

Project description:The impact of conditional Opa1 Knockout

| PRJNA976304 | ENA

Proteomic analysis of UCHL1 conditional knockout mice

Project description:Proteomic analysis of osteoclast precursor cells of UCHL1 conditional knockout mice and wild-type mice was performed

2024-08-14 | PXD052578 |

Proteomic profiling of eIF3a conditional knockout mice

Project description:In this study, a Cre-loxP system was used to generate eIF3a conditional knockout mice. Tandem mass tag (TMT) labeling with LC-MS/MS analysis was used to identify differentially expressed proteins (DEPs) in fat, lungs, skin, and spleen tissue of the eIF3a knockout mice and controls. Bioinformatics analysis was then used to explore the functions and molecular signaling pathways of these protein landscapes.

2023-07-20 | PXD041416 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data