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Identification of Meiotic-P26-bound RNAs in cutlured Drosophila SL2 cells by iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation)

ABSTRACT: Identification of Meiotic-P26-bound RNAs in cutlured Drosophila SL2 cells by iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation)

PROVIDER: PRJNA638097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11952874.fastq.gz	Fastqsanger.gz
	SRR11952875.fastq.gz	Fastqsanger.gz
	SRR11952876.fastq.gz	Fastqsanger.gz
	SRR11952877.fastq.gz	Fastqsanger.gz
	SRR11952878.fastq.gz	Fastqsanger.gz

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Identification of Meiotic-P26-bound RNAs in cutlured Drosophila SL2 cells by iCLIP (individual-nucleotide resolution UV crosslinking and immunoprecipitation)

Project description:The TRIM-NHL protein Meiotic P26 (Mei-P26) governs gene expression and controls stem cell fate in Drosophila. Mei-P26 represses the translation of the key regulator protein Nanos and induces miRNA-dependent silencing to regulate stem cell renewal, differentiation and proliferation. The structural and mechanistic details of mRNA target recognition and RNA binding by its C-terminal NHL domain remain elusive. To identify its cellular target RNAs, we performed iCLIP2 experiments in cultured Drosophila SL2 cells that were transfected with Flag-tagged Mei-P26 full-length, or its NHL domain.

2021-06-01 | GSE152013 | GEO

Individual nucleotide resolution Crosslinking Immunoprecipitation (iCLIP)-seq

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Mapping the targeting transcripts and binding sites of ALKBH5 by individual-nucleotide resolution UV crosslinking and immunoprecipitation-based sequencing (iCLIP-seq)

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