Project description:Spindlin1 is a unique multivalent epigenetic reader that facilitates ribosomal RNA transcription. In this study, we first identified that C11orf84 is a Spindlin1 bona fide binding partner. We performed ChIP-seq in U2OS cells to examine the genome-wide distribution of Spindlin1 and C11orf84. The ChIP-seq data indicates there is approximately 90% overlap in Spindlin1 and C11orf84 target genes.
Project description:We report genome-wide distribution of O-GlcNAcylated H2A at serine 40 (H2A-S40Gc) in mouse trophoblast stem cells (TSCs). We found that H2A-S40Gc was mainly located at genic area, positively correlated with the gene expression, and varied the localization during their differentiation. This study using ChIP-seq analysis provides genomic distribution of newly O-GlcNAc histone modification.
Project description:We have identified ZNF618 as a novel binding partner of UHRF2. We have found that UHRF2 and ZNF618 co-localize at many genomic loci. Examination of genome-wide distribution of SFB-tagged UHRF2 and ZNF618 in 293T cells using ChIP-seq.
Project description:We report genome-wide distribution of Linker histone variant H1t in mouse spermatogenic cells. We found that H1t was mainly located at around transcriptional start site of genic area, positively correlated with the gene expression. This study using ChIP-seq analysis provides genomic distribution of H1t in mouse spermatogenic cells.