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ABSTRACT: miRNA changes in Pelteobagrus vachelli muscle and brain induced by hypoxia

PROVIDER: PRJNA643883 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12141624.fastq.gz	Fastqsanger.gz
	SRR12141625.fastq.gz	Fastqsanger.gz
	SRR12141626.fastq.gz	Fastqsanger.gz
	SRR12141627.fastq.gz	Fastqsanger.gz
	SRR12141628.fastq.gz	Fastqsanger.gz

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Project description:Transcriptomic changes in Pelteobagrus vachelli muscle and brain induced by hypoxia

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Conjoint analysis of transcriptional and protein changes in heterosis of hybrid yellow catfish Huangyou-1 uncovers key roles for miRNAs [miRNA-Seq]

Project description:In this study, we used next-generation sequencing technologies and tandem mass tags to characterize mRNA-seq, miRNA-seq and proteomic of Pelteobagrus fulvidraco, P. vachelli and hybrid yellow catfish Huangyou-1 (P. fulvidraco female ×P. vachelli male) livers and in doing so, offer deeper insight into the transcriptional and protein changes in heterosis uncovers key roles for miRNAs.

2019-06-12 | GSE118625 | GEO

Conjoint analysis of transcriptional and protein changes in heterosis of hybrid yellow catfish Huangyou-1 uncovers key roles for miRNAs [RNA-Seq]

Project description:In this study, we used next-generation sequencing technologies and tandem mass tags to characterize mRNA-seq, miRNA-seq and proteomic of Pelteobagrus fulvidraco, P. vachelli and hybrid yellow catfish Huangyou-1 (P. fulvidraco female×P. vachelli male) livers and in doing so, offer deeper insight into the transcriptional and protein changes in heterosis uncovers key roles for miRNAs.

2019-06-12 | GSE118626 | GEO

Integrated analysis of mRNA-seq and miRNA-seq in the liver of Pelteobagrus vachelli in response to hypoxia

Project description:we used next-generation sequencing technology to characterise mRNA-seq and miRNA-seq of control- and hypoxia-treated P. vachelli livers to elucidate the molecular mechanisms of hypoxia adaptation. We were able to find miRNA-mRNA pairs using bioinformatics analysis and miRNA prediction algorithms.

2016-12-20 | GSE75614 | GEO

Conjoint analysis of transcriptional and protein changes in heterosis of hybrid yellow catfish Huangyou-1 uncovers key roles for miRNAs

Project description:Heterosis is a complex biological phenomenon in which hybridization exhibit superior phenotypic characteristics. The underlying molecular basis for heterosis, particularly for fishes, remains elusive. In this study, we used next-generation sequencing technologies and tandem mass tags to characterise mRNA-seq, miRNA-seq and proteomic of Pelteobagrus fulvidraco, P. vachelli and hybrid yellow catfish Huangyou-1 (P. fulvidraco ♀×P. vachelli ♂) livers and in doing so, offer deeper insight into the transcriptional and protein changes in heterosis uncovers key roles for miRNAs.

2019-05-15 | PXD010728 |

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Project description:Integrated analysis of mRNA-seq and miRNA-seq in the liver of Pelteobagrus vachelli in response to hypoxia

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Project description:Transcriptional profiles of liver in Pelteobagrus vachelli

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The proteomic changes in Pseudobagrus vachelli muscle and brain induced by hypoxia

Project description:The present NGST, TMT and Q-TOF MS platform should provide unprecedented resources to address such questions as to how hypoxic condition affects gene, miRNA, protein, and metabolite expression and changes the molecular pathways, and whether miRNAs participate in this process. For this purpose, we characterise transcriptomic, miRNAomic, proteomic and metabonomic sequencing of control- and hypoxia-treated P. vachelli muscles to elucidate the molecular mechanisms of hypoxia adaptation. We were able to find the predicted miRNA-mRNA-protein-metabolite regulatory network using bioinformatics analysis and miRNA prediction algorithms (Fig. 1). This is the first report on integrated analysis of transcriptome, miRNAome, and proteome, and metabolome in fishes and as such offers deeper insight into the hypoxia molecular mechanisms. We provide a good case study with which to analyse mRNA, miRNA, protein and metabolite expression and profile non-model fish species.

2020-07-18 | PXD020425 |

Project description:16S rRNA gene sequencing of intestinal microflora in Pelteobagrus vachelli

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The parallel reaction monitoring assays in heterosis of hybrid yellow catfish Huangyou-1

Project description:Heterosis is a complex biological phenomenon in which hybridization exhibits superior phenotypic characteristics. The underlying molecular basis for heterosis, particularly for fishes, remains elusive. The utilization rate of fish heterosis far exceeds our understanding of it on a theoretical level. To gain a comprehensive and unbiased molecular understanding of fish heterosis, we characterized mRNA-seq, miRNA-seq, and proteomes of Pelteobagrus fulvidraco, P. vachelli, and the hybrid yellow catfish Huangyou-1 (P. fulvidraco ♀×P. vachelli ♂) livers using next-generation sequencing technologies and tandem mass tags. Our results show that the nonadditive, homoeologue expression bias and expression level dominance pattern were readily identified in transcriptional, post-transcriptional, or protein levels, providing the evidence for the widespread presence of dominant models during hybridization. At the same time, a number of miRNA-mRNA-protein pairs were found and validated by qRT-PCR and parallel reaction monitoring assays.

2019-04-26 | PXD011343 |

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