Project description:Screening of domesticated complex flora from wastewater can degrade high concentrations of thiocyanate, the molecular mechanism of this phenomenon remains unclear. In this study, macro-proteomics techniques were used to analyse the intracellular proteins of the composite flora. The researchers performed proteomic analysis using combined tandem mass spectrometry to identify the differential proteins of the colonies under glucose treatment and thiocyanate stress. A total of 4,747 differential proteins were detected across the three different periods. Of these, 1,213 differential proteins were detected in the pre-biodegradation period, 1,300 differential proteins in the mid-biodegradation period, and 1,413 differential proteins in the post-biodegradation period. Through the protein function annotation, it was found that the differential proteins were mainly concentrated in carbon metabolism, energy metabolism, two-component system and amino acid metabolism.
Project description:Anaerobic microbial communities play a critical role in biogeochemical cycles through complex networks of interactions that influence community structure, stability, and functionality. This study investigates the metabolic interactions within synthetic communities composed of a cellulolytic bacterium (Ruminiclostridium cellulolyticum), a hydrogenotrophic methanogen (Methanospirillum hungatei), an acetoclastic methanogen (Methanosaeta concilii), and a sulfate-reducing bacterium (Desulfovibrio vulgaris). Through proteogenomic analysis and metabolic modeling, we quantified the metabolic interaction potential and metabolic resource overlap across bi-, tri-, and quad-cultures.