Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mucin drives human gut microbial ecological interactions in an in vitro synthetic community

ABSTRACT: In this study, we created a synthetic mucin-degrading microbial community to specifically study mucin-driven ecological interactions in vitro. The synthetic community consisted of primary mucin degraders and cross-feeders. We tracked community composition and dynamics and the mucin-degrading enzymes that were produced.

INSTRUMENT(S):

ORGANISM(S): Bacteroides Caccae Atcc 43185 Agathobacter Rectalis Atcc 33656 Akkermansia Muciniphila Atcc Baa-835 [ruminococcus] Torques Atcc 27756 Anaerostipes Caccae Dsm 14662 Phocaeicola Vulgatus Atcc 8482 Anaerobutyricum Hallii Dsm 3353 Desulfovibrio Piger Atcc 29098 Bacteroides Fragilis (strain Atcc 25285 / Nctc 9343) Faecalibacterium Prausnitzii A2-165 Ruminococcus Gnavus Atcc 29149 Roseburia Intestinalis L1-82 Methanobrevibacter Smithii Dsm 11975 Blautia Hydrogenotrophica Dsm 10507 Bacteroides Thetaiotaomicron (strain Atcc 29148 / Dsm 2079 / Nctc 10582 / E50 / Vpi-5482)

SUBMITTER: Sjef Boeren

LAB HEAD: Sjef Boeren

PROVIDER: PXD051481 | Pride | 2025-03-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	MQ_result_RA503-518_RA568-583.zip	Other
	RA503_MDSC_t72_R1.raw	Raw
	RA504_MDSC_t72_R2.raw	Raw
	RA505_MDSC_t72_R3.raw	Raw
	RA506_MDSC_t96_R1.raw	Raw

Items per page:

1 - 5 of 34

Publications

Malpractice: A professional risk.

Perlis D W DW Brucker M C MC

Journal of nurse-midwifery 19830301 2

PMID: 6551437

Similar Datasets

Transcriptional profiling of C. albicans in the presence of mucin versus absence of mucin

Project description:Candida albicans is the most prevalent fungal pathogen of humans, causing a variety of diseases ranging from superficial mucosal infections to deep-seated systemic infections. Mucus, the gel that coats all wet epithelial surfaces, accommodates Candida albicans as part of the regular microbiome where C. albicans resides asymptomatically in healthy humans. Through a series of in vitro experiments combined with genome-wide transcriptional profiling, we show that mucin biopolymers, the main gel-forming constituents of mucus, induce a new oval-shaped morphology in C. albicans in which a range of genes related to adhesion, filamentation, and biofilm formation are down-regulated. We also show that corresponding phenotypes are suppressed, rendering Candida incapable of forming biofilms on a range of different synthetic surfaces and human epithelial cells. Our data suggests that mucins can manipulate Candida physiology and we hypothesize that they are key regulators for retaining Candida in the host-compatible, commensal state. 3 biological replicates grown in log phase in the presence and absence of mucin for 8 hours. Experiments were performed using RPMI 1640 (Gibco 31800-089) buffered with 165mM MOPS and supplemented with 0.2% NaHCO3 and 2% glucose with and without 0.5% mucin.

2014-03-11 | E-GEOD-55766 | biostudies-arrayexpress

Mucin type affects synthetic community composition

Project description:Mucin type affects synthetic community composition

| PRJEB83805 | ENA

MDSC

Project description:A mucin-degrading synthetic microbial community

| PRJEB72538 | ENA

Campylobacter jejuni Utilization of Mucin

Project description:While growing in the human intestine, C. jejuni grows within the mucus layer. The largest constituents of this layer are the large mucin glycoproteins. A transcriptomic profile of C. jejuni NCTC11168 growing in a mucin-containing minimal medium seeks to describe the effect of the presence of mucin proteins on the transcriptome of C. jejuni. Microarray data was collected from three independent biological replicates and 9 technical replicates for each biological replicate.

2011-04-01 | E-GEOD-28295 | biostudies-arrayexpress

Human milk oligosaccharide degradation by a defined consortium of the infant gut microbiota.

Project description:With this study, we wanted to investigate degradation of human milk oligosaccharides and the subsequent cross-feeding interactions of a complex bacterial community that resembles the gut microbiota of pre-weaned vaginally-born breastfed infants.

2024-08-27 | PXD047785 | Pride

Human plasma proteins detected by HPLC-MS/MS after blood incubation with E. coli treated/not treated with mucin

Project description:Whole human blood incubated with E. coli isolate with or without bound mucin. Plasma was obtained and the effect of mucin bound to E. coli on plasma proteins content was analysed by HPLC-MS-MS.

2019-12-16 | PXD010496 | Pride

Mucin-binding proteins of E. coli membrane

Project description:Proteins from E. coli outer membrane were extracted with carbonate method, separated in 2D-PAGE and transferred onto nitrocellulose membrane. After incubation with flourescent-labelled mucin, mucin-binding protein spots were cut out, subjected to trypsinolysis and identified by protein analysis with HPLC-MS-MS.

2019-12-16 | PXD010472 | Pride

Ruminococcus torques is a keystone degrader of intestinal mucin glycoprotein, releasing oligosaccharides used by Bacteroides thetaiotaomicron

Project description:Symbiotic interactions between humans and our communities of resident gut microbes (microbiota) play many roles in health and disease. Some gut bacteria utilize mucus as a nutrient source and can under certain conditions damage the protective barrier it forms, increasing disease susceptibility. We investigated how Ruminococcus torques—a known mucin-degrader that remains poorly studied despite its implication in inflammatory bowel diseases (IBDs)— degrades mucin glycoproteins or their component O-linked glycans to understand its effects on the availability of mucin-derived nutrients for other bacteria. We found that R. torques utilizes both mucin glycoproteins and released oligosaccharides from gastric and colonic mucins, degrading these substrates with a panoply of mostly constitutively expressed, secreted enzymes. Investigation of mucin oligosaccharide degradation by R. torques revealed strong fucosidase, sialidase and b1,4-galactosidase activities. There was a lack of detectable sulfatase and weak β1,3-galactosidase degradation, resulting in accumulation of glycans containing these structures on mucin polypeptides. While the Gram-negative symbiont, Bacteroides thetaiotaomicron grows poorly on mucin glycoproteins, we demonstrate a clear ability of R. torques to liberate products from mucins, making them accessible to B. thetaiotaomicron. This work underscores the diversity of mucin-degrading mechanisms in different bacterial species and the probability that some species are contingent on others for the ability to more fully access mucin-derived nutrients. The ability of R. torques to directly degrade a variety of mucin and mucin glycan structures and unlock released glycans for other species suggests that it is a keystone mucin degrader, which may contribute to its association with IBD.

2024-06-19 | GSE262875 | GEO

Mucolytic activity of Akkermansia muciniphila enables Human Milk Oligosaccharides degradation.

Project description:This study was conducted in order to monitor whether or not Akkermansia muciniphila was able to grow and utilize human milk and human milk oligosaccharides by deploying its mucin degrading enzymes. Interestingly, A. muciniphila was able to grow in human milk producing Short Chain Fatty Acids and degrade milk oligosaccharides (2’-fucosyllactose, 3’-siallylactose) as well as lactose.

2020-09-07 | PXD011357 | Pride

MDSC_NOAm

Project description:Niche occupation by Akkermansia muciniphila in a synthetic mucin-degrading community

| PRJEB72622 | ENA