Project description:Microarray analysis was performed in order to detail the gene expression profiles in murine b-2M-SPa-6+c-kit-undifferentiated and b-2M-SPa-6+c-kit+ differentiating spermatogonia. These data were used to compare human and mouse transcriptomes of undifferentiated spermatogonia.
Project description:ID4 belongs to a family of transcriptional regulators involved in stem cell function. ID family proteins act by binding and inhibiting activity of basic-HLH (bHLH) transcription factors, including E-proteins and class II factors. ID function is also mediated through interaction with non-bHLH proteins. Spermatogonial stem cells (SSCs) are found within a population of undifferentiated spermatogonia in the testis and are essential for the sustained production of sperm over the lifespan. It is reported that ID4 is involved in SSC maintenance but the downstream targets of ID4 in SSCs are unclear. To gain insight into the function of ID4 in SSCs we identified interacting partners in cultures of mouse undifferentiated spermatogonia by immunoprecipitation using a specific ID4 antibody and mass spectrometry analysis.
Project description:To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads. We then performed proteomic analysis using protein lysates obtained from THY1 + spermatogonia.
Project description:Full title: Prepubertal Human Spermatogonia and Mouse Gonocytes Share Conserved Gene Expression of Germline Stem Cell Regulatory Molecules Oligonucleotide Microarray Analysis Reveals Novel Genes Expressed in Prepubertal Human Spermatogonia and Mouse Gonocytes Four hundred cells were selected for each group of germ cells or testis somatic cells from three independent testis cell preparations from both human (age 2, 8 and 10 yr) and mouse (age all 3 days) Selected germ and somatic cells were placed in 25 µl Trizol reagent (Invitrogen) immediately after isolation Oligonucleotide microarray was utilized to identify genes enriched in human and mouse selected germ cell populations and testis somatic cells.