Project description:The study aims to elucidate the effect of histone methyltransferase SMYD3 on gene expression in MCF-7 breast cancer cell line. Knockdown luciferase control v.s. knockdown SMYD3 in MCF-7 breast cancer cell line were conducted. Results identify a large proportion of cell cycle-related genes regulated by SMYD3.
Project description:N-terminal peptide enrichment for ATE1 knockdown mutant breast cell line. Immortalized human breast cell line HMEC, and breast cancer cell line T47D were used.
Project description:Human breast cancer cell line MDA-MB-231 was transduced with lentivirus vector expressing non-specific control (NS) and CFH knockdown (shCFH1) sequences.