Transcriptional effects of LXR nuclear receptors on GM-CSF-derived macrophages
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ABSTRACT:
PROVIDER: PRJNA658788 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12502806.fastq.gz | Fastqsanger.gz | |||
| SRR12502807.fastq.gz | Fastqsanger.gz | |||
| SRR12502808.fastq.gz | Fastqsanger.gz | |||
| SRR12502809.fastq.gz | Fastqsanger.gz | |||
| SRR12502810.fastq.gz | Fastqsanger.gz |
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Project description:Analysis of the role of LXR transcriptions factors on the transcriptional signature of human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were treated with LXR agonist GW3965 (1 uM, Tocris), LXR antagonist GSK2033 (1 uM, Tocris), GW3965 and GSK2033, or dimethyl sulfoxide (DMSO) as vehicle. In the dual condition, the antagonist was added 1-hour prior to agonist treatment. Then, monocytes were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days.
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