Ontology highlight
ABSTRACT:
PROVIDER: PRJNA660619 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR12562768_1.fastq.gz | Fastqsanger.gz | |||
| SRR12562768_2.fastq.gz | Fastqsanger.gz | |||
| SRR12562769_1.fastq.gz | Fastqsanger.gz |
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Project description:Objectives: To determine the transcripts that are differentially expressed in a hfq mutant. Hfq is an RNA chaperone that mediates many interactions between regultory RNAs and their mRNA targets. Analysis of the transcriptomes of the Pasteurella multocida wild-type strain and the Pasteurella multocida hfq mutant will allow for identification of genes controlled by hfq and the sRNAs with which it interacts. Methods: RNA sequencing was employed to determine the transcriptomes of a wild-type Pasteurella multocida strain and a hfq mutant strain. Comparison of these two transcriptomes allows for determination of differentially expressed genes and therefore those genes controlled by Hfq and sRNAs with which it interacts.
2016-03-02 | E-GEOD-77721 | biostudies-arrayexpress
Project description:Objectives: To determine the transcripts that are differentially expressed in a hfq mutant. Hfq is an RNA chaperone that mediates many interactions between regultory RNAs and their mRNA targets. Analysis of the transcriptomes of the Pasteurella multocida wild-type strain and the Pasteurella multocida hfq mutant will allow for identification of genes controlled by hfq and the sRNAs with which it interacts.
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Project description:Quantitative proteomics comparison wt and Hfq mutant Pasteurella multocida strains.
2016-03-14 | PXD003586 | Pride
Project description:Analysis of macrolide-resistant porcine Pasteurella multocida isolates from Germany
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Project description:Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in control (cellulose) and garlic-fed C57BL/6J mice serendipitously infected with Pasteurella multocida and identified acute phase response genes, particularly Lcn2 and Orm2, as the major players in the innate response. Also dieraty garlic suppressed pasteurella infection in C57BL/6J mice.
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Project description:Treatment of bacteria with antibiotics at or close to the inhibitory concentration leads to specific transcriptional responses often affecting target genes and targets pathways. A dataset of transcriptional profiles (compendium) induced by antibiotics with known mode-of-action (MoA) can be used to gain information on the putative MoA of novel substances with unknown MoAs. We used a Pasteurella multocida microarray to generate a compendium of transcriptional profiles and to obtain information on the putative MoA of a novel antibiotic compound. We also show a strong impact of the bacteriostatic antibiotics on P. multocida virulence gene transcription. Keywords: antibiotica treatment, time course Midlog-grown cultures of P. multocida were treated for 10 or 30 min with 8 different antibiotics and one novel compound (thiazin) at minimal inhibitory concentrations (MICs) and were harvested. Control bacteria were not-treated and harvested at approximately the same optical density an OD578 of ~ 0.5. Total RNA was extracted from these samples and labelled with biotin. P. multocida whole genome transcriptional profiling was performed by hybridization on the custom-made Affymetrix microarray according to the manufacturerâs instructions. The experiments were done in triplicates.
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Project description:Treatment of bacteria with antibiotics at or close to the inhibitory concentration leads to specific transcriptional responses often affecting target genes and targets pathways. A dataset of transcriptional profiles (compendium) induced by antibiotics with known mode-of-action (MoA) can be used to gain information on the putative MoA of novel substances with unknown MoAs. We used a Pasteurella multocida microarray to generate a compendium of transcriptional profiles and to obtain information on the putative MoA of a novel antibiotic compound. We also show a strong impact of the bacteriostatic antibiotics on P. multocida virulence gene transcription. Keywords: antibiotica treatment, time course
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Project description:Pasteurella multocida is a Gram-negative capsulated bacterium responsible for a range of diseases that cause severe morbidity and mortality in livestock animals. The hyaluronic acid (HA) capsule produced by P. multocida serogroup A strains is a critical virulence factor. In this study, we utilised transposon-directed insertion site sequencing (TraDIS) to identify genes essential for in vitro growth of P. multocida, and combined TraDIS with discontinuous density gradients (TraDISort) to identify genes required for HA capsule production and regulation in this pathogen. Analysis of mutants with a high cell density phenotype, indicative of the loss of extracellular capsule, led to the identification of 69 genes important for capsule production. These genes included all previously characterized genes in the capsule biosynthesis locus, and fis and hfq that encode known positive regulators of P. multocida capsule. Many of the other capsule-associated genes identified in this study were involved in regulation or activation of the stringent response, including spoT and relA that encode proteins that regulate the concentration of guanosine alarmones. Disruption of the autoregulatory domains in the C-terminal half of SpoT using insertional mutagenesis resulted in reduced expression of capsule biosynthesis genes and an acapsular phenotype. Overall, these findings have greatly increased the understanding of hyaluronic acid capsule production and regulation in P. multocida.
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Project description:Pasteurella multocida is a pathogen that causes a range of distinct diseases in livestock animals. Different P. multocida strains produce different capsule and lipopolysaccharide (LPS) structures. Different P. multocida diseases are associated with different capsule and LPS types, and little is known about what underpins this disease specificity. In this study, we utilised transposon-directed insertion site sequencing (TraDIS, also called Tn-Seq) to identify genes required for growth in rich media, and genes required for survival during systemic infections in BALB/c mice, for two diverse P. multocida strains, strain VP161 (capsule type A and LPS type L1) and strain M1404 (capsule type B and LPS type L2). Rich media analysis showed that both VP161 and M1404 shared 461 genes essential for growth in rich media, with comparison to the entire species showing that 95% of these rich media essential genes present in all publicly available closed P. multocida genomes. In vivo fitness analysis identified 63 and 94 genes important for VP161 and M1404 survival in BALB/c mice, respectively. Only 35 homologs were identified in both strains as important for survival, showing that conserved biological systems can be differentially important for different P. multocida strains. Investigation of proteins involved in the catabolite response showed that an active cyclic-adenosine monophosphate (cAMP) receptor protein (CRP) was required for maximal fitness in M1404. Furthermore, disrupting CRP or cAMP production also reduced capsule production in M1404, but increased capsule production in VP161, showing different strains of P. multocida have different regulatory systems for crucial virulence factors.
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