Cell Fate Determining Molecular Switches and Signaling Pathway in Pax7-expressing Somitic Mesoderm
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ABSTRACT:
PROVIDER: PRJNA666903 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12760000_1.fastq.gz | Fastqsanger.gz | |||
| SRR12760000_2.fastq.gz | Fastqsanger.gz | |||
| SRR12760100_1.fastq.gz | Fastqsanger.gz | |||
| SRR12760100_2.fastq.gz | Fastqsanger.gz | |||
| SRR12760200_1.fastq.gz | Fastqsanger.gz |
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Project description:During development, different cell types are originated from a common progenitor at a specific time frame. Previous lineage tracing of Pax7+ progenitors in somitic mesoderm has built the foundation of its development trajectory towards dermis, brown adipocytes, and skeletal muscle at dorsal trunk. Yet the molecular switches and mechanisms of such differentiation remain unknown. By combining inducible lineage tracing and single-cell RNA sequencing, here we show the transcriptomic profile of the Pax7 lineage, precise differentiation time point, and transcription factors that could drive lineage-specific differentiation. Utilizing surface markers identified in each cell types, we successfully enriched brown adipocytes, dermal fibroblasts, and cell-type specific progenitors for in vitro culture. Further characterization has shown the importance of Wnt5a and Rgcc for lineage development during embryogenesis. We also propose eFAP (embryonic fibro/adipogenic progenitor) that resembles adult FAPs lineage potentials. The work provides further understanding of the Pax7 lineage during embryonic development.
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Project description:During development, different cell types originate from a common progenitor at well-defined time points. Previous lineage-tracing of Pax7+ progenitors from the somitic mesoderm has established its developmental trajectory towards the dermis, brown adipocytes, and skeletal muscle in the dorsal trunk; yet the molecular switches and mechanisms guiding the differentiation into different lineages remain unknown. We performed lineage-tracing of Pax7-expressing cells in mouse embryos at E9.5 and profiled the transcriptomes of Pax7-progenies on E12.5, E14.5, and E16.5 at single-cell level. Analysis of single-cell transcriptomic data at multiple time points showed temporal-specific differentiation events toward muscle, dermis, and brown adipocyte, identified marker genes for putative progenitors and revealed transcription factors that could drive lineage-specific differentiation. We then utilized a combination of surface markers identified in the single-cell data, Pdgfra, Thy1, and Cd36, to enrich brown adipocytes, dermal fibroblasts, and progenitors specific for these two cell types at E14.5 and E16.5. These enriched cell populations were then used for further culture and functional assays in vitro, in which Wnt5a and Rgcc are shown to be important factors that could alter lineage decisions during embryogenesis. Notably, we found a bipotent progenitor population at E14.5, having lineage potentials towards both dermal fibroblasts and brown adipocytes. They were termed eFAPs (embryonic fibro/adipogenic progenitors) as they functionally resemble adult fibro/adipogenic progenitors. Overall, this study provides further understanding of the Pax7 lineage during embryonic development using a combination of lineage tracing with temporally sampled single-cell transcriptomics.
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