Project description:Purpose: The goal of this study is to identify differentially expressed microRNAs in pry-1/Axin mutant compare to N2 wild-type (WT). Our study represents the first microRNA analysis of Axin transcriptome in C. elegans and facilitates investigations of axin mediated processes.
Project description:Germ granules are RNA-rich biomolecular condensates regulating posttranscriptional gene expression. In C. elegans, germ granules are associated with the NPC. Mechanism by which germ granules associate with the nucleus and relevant functions remain unknown. We uncovered the function of the NPP-14/24 complex in spatially organizing perinuclear germ granules and fine-tuning the piRNA-based transcriptome surveillance and the inheritance of gene silencing.
Project description:We sequenced the transcriptome of a host (Caenorhabditis elegans) following its interaction with a non-native bacterium (Enterococcus faecalis) that has protective traits against the pathogen, Staphylococcus aureus. We also investigated the impact that the evolutionary history of the protective bacterium has on the transcriptional history of the host. We tested protective bacteria that had either coevolved against the pathogen within C. elegans, or had evolved on its own within C. elegans.
