Project description:In macrophage biology, resident peritoneal macrophages (RPMs) and thioglycolate-elicited peritoneal macrophages (TGPMs) have been traditionally utilized as primary cultured models. RPMs and TGPMs display distinct morphological and functional characteristics. We analyzed altered gene expression between RPMs from control mice and TGPMs from thioglycolate-injected mice.
Project description:Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. We resolved a restricted transcriptional profile for the self-renewing population of peritoneal resident macrophages, which is expressed during homeostasis and inflammation and distinct from other MM-CM-^X. Prominent within this profile was the expression of Gata6. This study represents a characterisation of the role of Gata6 in peritoneal resident macrophage phenotype. We used microarrays to determine the patterns of gene expression in peritoneal resident MM-CM-^X in the absence of GATA-6 against wild type. Conditional 'floxed' Gata6 deficient sex-matched mice between 7 weeks old were compared against wild type
Project description:microRNA transcriptome data from wild type and Gata6-deficient tissue resident peritoneal macrophages. Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. Gata6 itself has been shown to be a target of multiple miR. However, microRNA transcriptome and its dependence on tissue-specific macrophage programming, such as effected by GATA6, has not been explored. We used microRNA sequencing to determine the patterns of microRNA expression in peritoneal resident macrophages at homeostasis in the absence of GATA-6 against wild type.
Project description:RNA transcriptome data from C57BL/6 tissue resident peritoneal macrophages over expressing microRNA 708 or control. The role of microRNA-708 in shaping macrophage biology remains mostly unknown. Here, using lentiviral vectors we overexpressed microRNA-708 in vivo in C57BL/6 mice peritoneal macrophages and investigated mRNA changes in these cells after 4 days.
Project description:Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. We resolved a restricted transcriptional profile for the self-renewing population of peritoneal resident macrophages, which is expressed during homeostasis and inflammation and distinct from other MØ. Prominent within this profile was the expression of Gata6. This study represents a characterisation of the role of Gata6 in peritoneal resident macrophage phenotype. We used microarrays to determine the patterns of gene expression in peritoneal resident MØ in the absence of GATA-6 against wild type.
Project description:Resident CD102+ peritoneal and plueral macrophages were FACS-sorted from unmanipulated male and female C57BL/6 mice and compared by RNAseq.
Project description:Here we study the effect of LPS in the transcriptome of thioglycollate-elicited peritoneal macrophages isolated from Ldlr knock out mice
Project description:Activation of signaling pathways downstream of Toll-like receptor 4 upregulate expression of genes related to serine metabolism. We cultured peritoneal macrophages in control or serine/glycine-depleted medium for 24 hr, stimulated them with LPS (10 ng/ml) for 6 or 12 hr, and collected them to compare the gene expression. Microarray analysis revealed that serine/glycine-depletion in peritoneal macrophages upregulates gene expression of enzymes for serine biosynthesis.
Project description:Research based essentially on mouse models of ovarian cancer peritoneal metastasis have revealed that peritoneal macrophages, that include resident peritoneal macrophages (resMØs) and monocyte-derived non-resident macrophages, promote peritoneal tumor growth, through the induction of tumor cell proliferation and migration, and immunosuppression. However, the mechanisms controlling the expansion and differential contribution of resMØs and monocyte-derived non-resident macrophages to the pool of peritoneal macrophages present in tumor-bearing mice, and to promote tumor growth, remains to be addressed. Using a mouse model of colorectal cancer (CRC) peritoneal metastasis, based on the intraperitoneal injection of mouse tumor organoids, derived from primary tumors arising in genetically engineered mice, bearing mutations in Apc, Kras, Tgfbr2 and Trp53, and developing human-like metastatic CRC tumor growth, in the present report, the origin, expansion and function of peritoneal macrophages during peritoneal CRC tumor growth, has been investigated. Our data support that the low inflammatory status of the peritoneal cavity associated to CRC peritoneal tumor growth, restrained Ly6Chi monocyte recruitment and formation of Tim4- resMØs and moMØs, while enabling a marked cell proliferation-driven expansion of Tim4+ resMØs. Tumor-induced Tim4+ resMØs displayed a migratory and protumor transcriptomic signature, involving the activation of genes controlling the expression of important protumor molecules, such as A2A/A2B, ARG1, IDO, IL-6, IRG1, MMP12, PD-L1/2, SPP1, TREM1 and VEGFa. Correspondingly, during peritoneal CRC tumor growth, Tim4+ TREM1+ resMØs migrated to the omentum, the main peritoneal target organ for peritoneal metastasis, and promoted CRC peritoneal tumor progression.
Project description:Here we study the effect of LPS in the transcriptome of thioglycollate-elicited peritoneal macrophages isolated from Ldlr knock out and Ch25h;Ldlr double knock out mice