Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Single-cell Transcriptomic Atlas of Human Intervertebral Disc

ABSTRACT: A Single-cell Transcriptomic Atlas of Human Intervertebral Disc

PROVIDER: PRJNA674356 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12973953.fastq.gz	Fastqsanger.gz
	SRR12973954.fastq.gz	Fastqsanger.gz
	SRR12973955.fastq.gz	Fastqsanger.gz
	SRR12973956.fastq.gz	Fastqsanger.gz
	SRR12973957.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 40

Similar Datasets

A Single-cell Transcriptomic Atlas of Human Intervertebral Disc

Project description:Using high-precision transcriptomics to systematically map the atlas of human intervertebral disc (IVD) at single-cell resolution. Consequently, we found remarkable cellular diversity in the human IVD and identified a set of signature markers to recognize the cell types spatially. Furthermore, we deciphered a biological classification of chondrocyte subclusters with distinct role in the ECM homeostasis.Notably, the critical clues were also discovered for progenitor cells with bi-lineage differentiation trajectories in the nucleus pulposus, which discriminatively marked by the ancestry molecules PDGFRA and PROCR and highly enriched PDGF network. Finally, we uncovered the potential vital factors maintaining the IVD homeostasis from the intercellular crosstalk based on the signaling network landscape of the IVD microenvironment.

2021-08-20 | GSE160756 | GEO

Spatiotemporal characterization of human early intervertebral disc formation at single-cell resolution

Project description:Intervertebral disc (IVD) performs as a fibrocartilaginous joint to anchor adjacent vertebrae (VB) and is essential for the mechanical stabilization of axial skeleton. Although several studies have demonstrated the cellular heterogeneity of adult mature IVD, a single-cell transcriptomic atlas mapping early IVD formation is still lacking. Here, we generated the first spatiotemporal and single-cell based transcriptomic atlas of human IVD formation at embryonic stage, as well as a comparative mouse transcript landscape. The notochord (NC)/nucleus pulposus (NP) cells, vertebral chondrocytes, and annulus fibrosus (AF) cells were characterized. Our single-cell transcriptomic atlas that resolves spatiotemporally regulated cellular heterogeneity underlying early IVD development will be leveraged to develop preventative and regenerative strategies for IVD degeneration.

2025-10-25 | GSE216511 | GEO

Intervertebral disc degeneration

Project description:Intervertebral disc degeneration

| PRJNA962588 | ENA

Sox9 Deletion Causes Severe Intervertebral Disc Degeneration Characterized by Apoptosis, Matrix Remodeling, and Compartment-Specific Transcriptomic Changes

Project description:Transcriptomic analysis of nucleus pulposus (NP) and annulus fibrosus (AF) tissues from intervertebral discs of 3-month-old mice with the conditional postnatal deletion of Sox9. The transcription factor Sox9 is essential for the maintenance and health of the intervertebral disc of mice. We examined the transcriptomic profiles of nucleus pulposus and annulus fibrosus cells in control and Sox9 conditional knock-out mice using microarrays.

2023-05-19 | GSE150850 | GEO

Neonatal and Adult Human Intervertebral Disc scRNA-seq

Project description:We report the single-cell RNA-seq (scRNA-seq) data for human neonatal and adult human intervertebral disc (IVD) scRNA-seq. We sequenced cells harvested from three IVDs of a neonatal baby and one IVD from an adult cadaver.

2021-12-07 | GSE189916 | GEO

Integrated with Proteome-sequencing, bulk RNA-sequencing and Single cell RNA-sequencing to identify potential biomarkers in different grades of intervertebral disc degeneration

Project description:We selected humann intervertebral disc samples to perform proteomics analysis. There were 1 case of grade I , 1 case of grade II, 3 cases of grade Ⅲ and 3 cases of grade Ⅳ according to Pfirrmann classfication. RNA seqencing analysis and single-cell RNA sequencing were integrated with proteomics data to identify the hub genes for intervertebral disc degeneration using bioinformatic method.

2023-05-10 | PXD040593 | Pride

Single-cell sequencing reveals cellular heterogeneity of nucleus pulposus in intervertebral disc degeneration

Project description:Nucleus pulposus (NP) plays a vital role in intervertebral disc degeneration (IVDD). Previous studies have revealed cellular heterogeneity in the NP tissue during IVDD progression. Here, we used single cell RNA sequencing (scRNA-seq) to analyze the cellular and molecular alterations of diverse cell clusters during IVDD.

2024-11-14 | GSE251686 | GEO

Proteomics for patients with intervertebral disc degeneration

Project description:Proteomics for patients with intervertebral disc degeneration

2024-04-10 | PXD051351 |

Multi-omics Analysis and Evidence of IL1R1 as a Potential Biomarker for Diabetes-related Intervertebral Disc Degeneration

Project description:This study employs 10x Genomics single-cell RNA sequencing (scRNA-seq) to profile cellular changes within the intervertebral discs of diabetic mice. Diabetes mellitus is a systemic inflammatory disease known to drive pathological alterations in multiple organ systems. Here, we specifically investigate the process of diabetes-induced intervertebral disc degeneration. Our findings aim to provide novel insights into the pathogenic mechanisms underlying disc degeneration.

2025-10-27 | GSE305927 | GEO

Lactate Efflux From Intervertebral Disc Cells Is Required for Maintenance of Spine Health

Project description:Monocarboxylate Transporter 4 (MCT4) is important for H+/lactate efflux from nucleus pulposus cells in the intervertebral disc of mice. We examined the transcriptomic profile of nucleus pulposus cells in wild-type and MCT4 global knock-out mice using microarrays.

2020-05-15 | GSE130561 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data