Project description:We isolated a bacterial identified as the genus of Mycetocola from the infected Flammulina velutipes samples collected from mushroom house in Hebei, North China. The stipe of F. velutipes appeared water-soaked, soften and browning after infected, finally, the whole plant rotten. The incidence of the disease can reach more than 80% in the serious areas, resulting in an obvious decline in the yield and quality of the F. velutipes. Furthermore, the pathogenic bacteria may occurrence in large areas by accumulated in the annual cultivation process. Therefore, the most effective measures to control this bacterial disease is screening resistant strains of F. velutipes with high yield and good agronomic traits. We obtained a white F. velutipes strain JK502 which has strong resistance to this bacterium by using the double-layer plate method combined with the sporozoite hybridization. JK501, which can early fruited, was screened using the same parent SH9 by multi spore hybridization. In order to investigate the differences of gene expression between the parent strain and the selected strains, we completed the transcriptome sequencing by Illumina HiSeq X system.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Hebeloma cylindrosporum ectomycorrhizal root tips compared to free-living mycelium . Ectomycorrhizal root tips and control mycelium were harvested after 3 weeks and used for RNA extraction. Reads of 150 bp were generated and aligned to Hebeloma cylindrosporum transcripts (http://genome.jgi-psf.org/Hebcy2) using CLC Genomics Workbench 7.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Hebeloma cylindrosporum ectomycorrhizal root tips compared to free-living mycelium . Ectomycorrhizal root tips were harvested after 6 months and used for RNA extraction. Reads of 100 bp were generated and aligned to Hebeloma cylindrosporum transcripts (http://genome.jgi-psf.org/Hebcy2) using CLC Genomics Workbench 6.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Hebeloma cylindrosporum ectomycorrhizal root tips compared to free-living mycelium . Ectomycorrhizal root tips and control mycelium were harvested after 3 weeks and used for RNA extraction. Reads of 150 bp were generated and aligned to Hebeloma cylindrosporum transcripts (http://genome.jgi-psf.org/Hebcy2) using CLC Genomics Workbench 7. mRNA profiles from Hebeloma cylindrosporum ectomycorrhizal root tips and free-living mycelium were generated by Illumina HiSeq2000 sequencing (150bp). Three biological replicates were sequenced for mycorrhizal and mycelium samples.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Hebeloma cylindrosporum ectomycorrhizal root tips compared to free-living mycelium . Ectomycorrhizal root tips were harvested after 6 months and used for RNA extraction. Reads of 100 bp were generated and aligned to Hebeloma cylindrosporum transcripts (http://genome.jgi-psf.org/Hebcy2) using CLC Genomics Workbench 6. mRNA profiles from Hebeloma cylindrosporum ectomycorrhizal root tips and free-living mycelium were generated by Illumina HiSeq2000 sequencing (100bp). Ttwo biological replicates were sequenced for mycorrhizal and mycelium samples.
Project description:Illumina HiSeq2000 technology was used to generate mRNA profiles from Hebeloma cylindrosporum pre-infectious stages (2 and 4 days) compared to control mycelium. Pre-infectious and control mycelia were harvested after 2 and 4 days and used for RNA extraction. Reads of 150 bp were generated and aligned to Hebeloma cylindrosporum reference transcripts (http://genome.jgi-psf.org/Hebcy2) using CLC Genomics Workbench 8.5.1.