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Single-Cell RNA-seq Transcriptome Profiling from Male Germ Cells Isolated from Neonatal Mouse Testes

ABSTRACT: Single-Cell RNA-seq Transcriptome Profiling from Male Germ Cells Isolated from Neonatal Mouse Testes

PROVIDER: PRJNA682448 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13195628.fastq.gz	Fastqsanger.gz
	SRR13195629.fastq.gz	Fastqsanger.gz
	SRR13195630.fastq.gz	Fastqsanger.gz
	SRR13195631.fastq.gz	Fastqsanger.gz
	SRR13195632.fastq.gz	Fastqsanger.gz

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Single-Cell RNA-seq Transcriptome Profiling from Male Germ Cells Isolated from Late Fetal and Neonatal Mouse Testes

Project description:Single-Cell RNA-seq Transcriptome Profiling from Male Germ Cells Isolated from Late Fetal and Neonatal Mouse Testes

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Single-Cell RNA-seq Transcriptome Profiling from Male Germ Cells Isolated from Late Fetal and Neonatal Mouse Testes

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Single cell RNA-sequencing of testes from control and Brca1 germ cell-specific knockout mice

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Adjudin-induced germ cell loss from adult rat testes

Project description:This is a study to explore the transcriptional changes after Adjudin treatment in adult rat testes at three time points (control, 8 hour and 4 day). Adjudin, [1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide], is a potential male contraceptive that targets the Sertoli-germ cell interface and causes germ cell depletion from the seminiferous epithelium. Adjudin has been proved to be a useful model to study the mechanisms that regulate junction restructuring in the testis. Keywords: Sertoli-germ cell adhesion regulation using an Adjudin model

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DICER-interacting partners in juvenile mouse testes

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Project description:Mammalian male germ-cell development consists of three distinct phases: primordial germ cell (PGC) development, male germ-cell specification for spermatogonium development, and ensuing spermatogenesis. Here, we show an in vitro reconstitution of whole male germ-cell development by pluripotent stem cells (PSCs). Mouse embryonic stem cells (mESCs) are induced into PGC-like cells (mPGCLCs), which are expanded for epigenetic reprogramming. In reconstituted testes under an optimized condition, such mPGCLCs differentiate into spermatogonium-like cells with proper developmental transitions, gene expression, and cell-cycle dynamics and are expanded robustly as germline stem cell-like cells (GSCLCs) with an appropriate androgenetic epigenome. Importantly, GSCLCs show vigorous spermatogenesis, not only upon transplantation into testes in vivo but also under an in vitro culture of testis transplants, and the resultant spermatids contribute to fertile offspring. By uniting faithful recapitulations of the three phases of male germ-cell development, our study creates a paradigm for the in vitro male gametogenesis by PSCs.

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