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Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets II

ABSTRACT: Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets II

PROVIDER: PRJNA682615 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13199640.fastq.gz	Fastqsanger.gz
	SRR13199641.fastq.gz	Fastqsanger.gz

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Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets

Project description:Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets

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Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets I

Project description:Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets I

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Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets.

Project description:Tissue-resident memory T (TRM) cells provide key adaptive immune responses in infection, cancer, and autoimmunity. However, transcriptional heterogeneity of human intestinal TRM cells remains undefined. Here, we investigate transcriptional and functional heterogeneity of human TRM cells through study of donor-derived TRM cells from intestinal transplant recipients. Single-cell transcriptional profiling identifies two transcriptional states of CD8+ TRM cells, delineated by ITGAE and ITGB2 expression. We define a transcriptional signature discriminating these populations, including differential expression of cytotoxicity- and residency-associated genes. Flow cytometry of recipient-derived cells infiltrating the graft, and lymphocytes from healthy gut, confirm these CD8+ TRM phenotypes. CD8+ CD69+CD103+ TRM cells produce interleukin-2 (IL-2) and demonstrate greater polyfunctional cytokine production, whereas β2-integrin+CD69+CD103- TRM cells have higher granzyme expression. Analysis of intestinal CD4+ T cells identifies several parallels, including a β2-integrin+ population. Together, these results describe the transcriptional, phenotypic, and functional heterogeneity of human intestinal CD4+ and CD8+ TRM cells.

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PD-1-stimulated T cell subsets are transcriptionally and functionally distinct

Project description:PD-1-stimulated T cell subsets are transcriptionally and functionally distinct

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Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets I

Project description:Tissue-resident memory T (TRM) cells provide key adaptive immune responses in infection, cancer, and autoimmunity. However transcriptional heterogeneity of human intestinal TRM cells remains undefined. Here, we investigated transcriptional and functional heterogeneity of human TRM cells through study of donor-derived intestinal TRM cells from intestinal transplant recipients. Single-cell transcriptional profiling identified two distinct transcriptional states of CD8+ TRM cells, delineated by ITGAE and ITGB2 expression. We defined a transcriptional signature discriminating these two CD8+ populations, including differential expression of cytotoxicity- and residency-associated genes. Flow cytometry of recipient-derived cells infiltrating the graft and lymphocytes from healthy gut confirmed the two CD8+ TRM phenotypes. CD103+ CD8+ TRM cells produced IL-2, and demonstrated greater polyfunctional cytokine production, while β2-integrin+ CD69+ CD103- TRM cells had higher granzyme expression. Phenotypic and functional analysis of intestinal CD4+ T cells identified many parallels, including a distinct β2-integrin+ population. Together, these results describe the transcriptional, phenotypic, and functional heterogeneity of human intestinal CD4+ and CD8+ TRM cells.

2020-12-05 | GSE162685 | GEO

Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets II

2020-12-05 | GSE162686 | GEO

Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-05 | GSE162687 | GEO

Human monocyte subsets are transcriptionally and functionally altered in aging in response to pattern recognition receptor agonists

Project description:Human monocyte subsets are transcriptionally and functionally altered in aging in response to pattern recognition receptor agonists

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Tissue-resident memory T cells in epicardial adipose tissue comprise transcriptionally distinct subsets that are modulated in atrial fibrillation.

Project description:Atrial fibrillation (AF) is the most common sustained arrhythmia and carries an increased risk of stroke and heart failure. Here we investigated how the immune infiltrate of human epicardial adipose tissue (EAT), which directly overlies the myocardium, contributes to AF. Flow cytometry analysis revealed an enrichment of tissue-resident memory T (TRM) cells in patients with AF. Cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) and single-cell T cell receptor (TCR) sequencing identified two transcriptionally distinct CD8+ TRM cells that are modulated in AF. Spatial transcriptomic analysis of EAT and atrial tissue identified the border region between the tissues to be a region of intense inflammatory and fibrotic activity, and the addition of TRM populations to atrial cardiomyocytes demonstrated their ability to differentially alter calcium flux as well as activate inflammatory and apoptotic signaling pathways. This study identified EAT as a reservoir of TRM cells that can directly modulate vulnerability to cardiac arrhythmia.

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PD-1-stimulated T cell subsets are transcriptionally and functionally distinct

Project description:Analysis of 3 subsets of primary human CD4+ T cells (naive, CM, EM) stimulated with anti-CD3, anti-CD28 and PD-L1/PD-L2 for 18 hours. we show that naive, EM, and CM T cell subsets had distinct gene expression profiles following PD-1 ligation.

2021-07-01 | GSE174860 | GEO

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