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Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR

ABSTRACT: Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR

PROVIDER: PRJNA685803 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13260968.fastq.gz	Fastqsanger.gz
	SRR13260969.fastq.gz	Fastqsanger.gz
	SRR13260970.fastq.gz	Fastqsanger.gz
	SRR13260971.fastq.gz	Fastqsanger.gz
	SRR13260972.fastq.gz	Fastqsanger.gz

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Similar Datasets

Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR

Project description:creatine regulon is small and electrophoretic mobility shift demonstrates specific CahR binding only at the creA promoter suggesting much of the regulon is due to induction by downstream products. This study identifies ligand specificity and provides biological function to a previously uncharacterized GATR, contributing to our understanding of bacterial organic nitrogen compound metabolism.

2020-12-17 | GSE163362 | GEO

Creatine utilization as a sole nitrogen source in <i>Pseudomonas putida</i> KT2440 is transcriptionally regulated by CahR.

Project description:Glutamine amidotransferase-1 domain-containing AraC-family transcriptional regulators (GATRs) are present in the genomes of many bacteria, including all Pseudomonas species. The involvement of several characterized GATRs in amine-containing compound metabolism has been determined, but the full scope of GATR ligands and regulatory networks are still unknown. Here, we characterize Pseudomonas putida's detection of the animal-derived amine compound creatine, a compound particularly enriched in muscle and ciliated cells by a creatine-specific GATR, PP_3665, here named CahR (Creatine amidohydrolase Regulator). cahR is necessary for transcription of the gene encoding creatinase (PP_3667/creA) in the presence of creatine and is critical for P. putida's ability to utilize creatine as a sole source of nitrogen. The CahR/creatine regulon is small, and an electrophoretic mobility shift assay demonstrates strong and specific CahR binding only at the creA promoter, supporting the conclusion that much of the regulon is dependent on downstream metabolites. Phylogenetic analysis of creA orthologues associated with cahR orthologues highlights a strain distribution and organization supporting probable horizontal gene transfer, particularly evident within the genus Acinetobacter. This study identifies and characterizes the GATR that transcriptionally controls P. putida's metabolism of creatine, broadening the scope of known GATR ligands and suggesting GATR diversification during evolution of metabolism for aliphatic nitrogen compounds.

| S-EPMC10228380 | biostudies-literature

Transcriptional network between pCAR1 and KT2440 chromosome

Project description:The completely sequenced catabolic plasmid pCAR1 of Pseudomonas resinovorans CA10 confers the ability to degrade carbazole upon the recipient strain Pseudomonas putida KT2440. To study the coordinated expression of pCAR1 with its host chromosome, transcriptome of the transconjugant strain KT2440(pCAR1) was analyzed at growth with carbazole as the sole carbon source compared to succinate as an alternative carbon source. Next, horizontal gene transfer of a plasmid potentially alters the transcriptome of its host chromosome. Transcriptome of the transconjugant strain KT2440(pCAR1) was compared to the parental strain KT2440 at the same growth conditions. Furthermore, pCAR1 encodes a possible global regulator designated ORF70, which belongs to the MvaT family of H-NS-like nucleoid-associated proteins in Pseudomonas. To investigate the regulon under the control of ORF70, transcriptome of KT2440(pCAR1deltaORF70) was compared to the parental strain KT2440(pCAR1) at the same growth conditions. Keywords: Comparative transcriptome analysis

2007-05-01 | GSE7650 | GEO

Transcriptional response of Candida albicans to the utilization of amino acids as the sole carbon source

Project description:Purpose of this study was to define the transcriptional response of Candida albicans to the utilization of amino acids as the sole carbon source (C-source) in order to study regulation of metabolic adaptation of the fungus to amino acid-rich media. Furthermore, potential regulatory activities of the transcription factor Stp2 exceeding the described role in amino acid-uptake have been investigated. Therefore, the SC5314 wild type and a deletion strain of Stp2 (stp2Δ) were incubated in medium containing either glucose (SD) or a mixture of proteinogenioc amino acids (SC) as the sole C-source and the cellular transcriptome defined using RNA-sequencing.

2025-10-07 | GSE277530 | GEO

Expression profile of Pseudomonas putida KT2440 growing in myristic acid

Project description:We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic pattern of P. putida KT2440 cultures using myristic acid as the sole carbon source at the exponential phase of growth compared with cultures grown with glucose as the sole carbon source. Thus, we can explore the expression of genes involved in fatty acid degradation.

2018-12-31 | GSE116756 | GEO

Transcriptional response of Candida albicans to the utilization of aromatic compounds as the sole carbon source

Project description:Main goal of this analysis was to idnetify metabolic adaptation processes in C. albicans when aromatic compounds, like catechol or hydroquinone are used as the sole carbon source (C-source) and how this is mediated on the transcriptional level. Further, a suspected regulatory activity of the transcription factors Stp2, a central regulator of amino acid metabolism, and Zcf25, a putative regulator of the oxoadipate pathway, on these processes was investigated. Therefore, the SC5314 wild type and single and dual knockout strains of the aforementioned regulators (stp2Δ, zcf25Δ, stp2Δ/zcf25Δ) were incubated in medium containing either glucose (SD), a mixture of proteinogenioc amino acids (SC), catechol (CAT) or hydroquinone (HQN) as the sole C-source and the cellular transcriptome investigated via RNA-Sequencing to define trasncriptional changes in response to the indicated different C-sources.

2025-10-07 | GSE277771 | GEO

KT2440 wt vs. mut agmR -M9 plus glucose chloramphenicol-free medium

Project description:DNA microarrays were carried out to compare the transcriptional profiles of P. putida KT2440 and its isogenic knock-out mutant in agmR gene (mut agmR) when grown in M9 medium with glucose as a sole carbon source and in absense of chloramphenicol or any other stressor compound.

2011-12-01 | E-MEXP-3203 | biostudies-arrayexpress

Transcriptomic analysis of Alishewanella species utilizing pectin as a sole carbon source

Project description:Comparative transcriptomic analysis of three Alishewanella species utilizing pectin as a sole carbon source

2013-03-29 | GSE45511 | GEO

Aspergillus nidulans ∆pkaA strain grown on avicel as sole carbon source

Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours

2015-07-15 | GSE70917 | GEO

Aspergillus nidulans strains TNO2a3, ∆snfA and ∆schA grown on avicel as sole carbon source

Project description:Transcriptome of A. nidulans TNO2a3, ∆snfA and ∆schA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours.

2013-07-16 | GSE47810 | GEO

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