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Study of the transcriptional effect of ANO1 knock-down in two head and neck squamous cell carcinoma cell lines by RNA-sequencing of samples cultured in three dimensional collagen type I

ABSTRACT: Study of the transcriptional effect of ANO1 knock-down in two head and neck squamous cell carcinoma cell lines by RNA-sequencing of samples cultured in three dimensional collagen type I

PROVIDER: PRJNA686985 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13282013.fastq.gz	Fastqsanger.gz
	SRR13282014.fastq.gz	Fastqsanger.gz
	SRR13282015.fastq.gz	Fastqsanger.gz
	SRR13282016.fastq.gz	Fastqsanger.gz
	SRR13282017.fastq.gz	Fastqsanger.gz

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Study of the transcriptional effect of ANO1 knock-down in two head and neck squamous cell carcinoma cell lines by RNA-sequencing of samples cultured in three dimensional collagen type I

Project description:ANO1 is located in the 11q13 region that is commonly amplified in head and neck squamous cell carcinoma. However, the exact mechanism of ANO1's involvement in oncogenesis and cancer proliferation remains unclear. In this study, we discovered novel genes which have an interplay in ANO1 cancer signalling pathways and were significantly differentially expressed upon ANO1 knockdown. All cell lines were cultured in three dimensional collagen I, a matrix that closely resembles tumor microenvironment. The aim of this study was to reveal novel mediators and pathways in ANO1 cancer cell signalling, and therefore gain insight on novel molecules that can present potent therapeutic targets.

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