Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of short time IL-4 stimulated bone marrow derived cells with or without Elp3

ABSTRACT: RNA sequencing of short time IL-4 stimulated bone marrow derived cells with or without Elp3

PROVIDER: PRJNA699533 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13631480.fastq.gz	Fastqsanger.gz
	SRR13631481.fastq.gz	Fastqsanger.gz
	SRR13631482.fastq.gz	Fastqsanger.gz
	SRR13631483.fastq.gz	Fastqsanger.gz
	SRR13631484.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

RNA sequencing of short time IL-4 stimulated bone marrow derived cells with or without Elp3

Project description:Comparison of transcriptome of 2 hours IL-4 treated bone marrow derived cells (BMDMs) from WT and Elp3 KO mice

2022-07-04 | GSE166169 | GEO

Quantitative proteomics analysis of Elp3 deficient bone marrow derived macrophages aggragates with or without IL-4 treatment

Project description:Alternative macrophages are critical in parasites defense, the resolution of inflammation and wound healing. RNA modifications, which are defined as post-transcriptional changes in the chemical composition of RNA molecules, are involved in the immune response.Among tRNA-modifying enzymes which target the tRNA anticodon, Elongator and Cytosolic thiouridylase (Ctu)-1/2 complexes exclusively modify the wobble uridine (U34) base in cytosolic tRNAs. However, the full picture of how mRNA translational factors maintain protein synthesis accuracy and co-translational protein folding are far from being fully understood.To address this question,we evaluated the role of Elp3 on protein in the IL4 induced alternative bone marrow derived macrophages (BMDMs). The BMDMs from widetype and Elp3 myeloid specific knockout mice were treated by IL-4 for 24h or not. Then the aggregates were isolated from these BMDMs and performed by MS.

2022-09-12 | PXD023588 | Pride

The proteomic of IL-4 treated BMDMs from Wt and Elp3 KO mice

Project description:This experiement aims to know what the differences of protein translation are in the bone marrow derived macro-phages(BMDMs) from WT mice and Elp3 KO mice. We treated the BMDMs with or without IL-4 for 4 hours.

2022-09-12 | PXD033962 | Pride

Ribosome profiling experiment in control or Elp3 depleted B16 melanoma cells

Project description:We report the application of ribosome profiling using high-throughput sequencing and total mRNA used for normalisation upon depletion of ELP3 protein in B16 melanoma cells.

2018-06-26 | GSE111891 | GEO

RNAseq of naive CD4 T cells deficient for Elp3 activated for 18h against WT control

Project description:CD4 T cell deficient for the Elp3 gene have a delayed cell cycle entry. We explored by RNAseq by which molecular pathway the absence of Elp3 delayed the cell cycle entry. Naive T cell from CD4-cre Elp3 fl/fl mice and WT littermate were purified and activated in vitro with CD3 and CD28 antibodies for 18h.

2020-07-31 | E-MTAB-9057 | biostudies-arrayexpress

RNAseq of follicular helper T cell from Elp3 deficient mice after Complete Freund Adjuvant immunisation

Project description:Loss of Elp3 in CD4 T cells hindered the development of an optimal T follicular helper T cell (TFH) response. To understand the molecular pathway by which Elp3 is necessary for TFH responses, mice deficient for Elp3 in T cells and WT littermate were immunized with ovalbumin emulsified with complete Freund Adjuvant. 8 days later, TFH were sorted from the draining lymph nodes and submitted for RNAseq

2020-07-31 | E-MTAB-9059 | biostudies-arrayexpress

Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer

Project description:Quantitative and qualitative changes in mRNA translation occur in tumor cells and support cancer progression and metastasis. Post-transcriptional nucleoside modifications of transfer RNAs (tRNAs) at the wobble U34 base are highly conserved and contribute to translation fidelity. Here, we show that ELP3 and CTU1/2, partner enzymes in U34 mcm5s2-tRNA modification, are upregulated in human breast cancers and sustain metastasis. Elp3 genetic ablation strongly impaired invasion and metastasis formation in the PyMT model of invasive breast cancer. Mechanistically, ELP3 and CTU1/2 support cellular invasion through the translation of the oncoprotein DEK. As a result, DEK promotes the IRES-dependent translation of the pro-invasive transcription factor LEF1. Consistently, a DEK mutant, whose codon composition is independent of U34 mcm5s2-tRNA modification, escapes the ELP3- and CTU1-dependent regulation and restores the IRES-dependent LEF1 expression. Our results demonstrate the key role of U34 tRNA modification to support specific translation during breast cancer progression and highlight a functional link between tRNA modification- and IRES-dependent translation during tumor cell invasion and metastasis.analysis of transcriptomic changes due to Elp3genetic deletion in cells extracted from PyMT mammary tumors.

2016-09-29 | GSE85810 | GEO

microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

Project description:Lipopolysaccharide stimulated primary human monocyte-derived macrophages at 2, 4 and 8hrs, IL-10 at 4hrs, IL-10+LPS at 4hrs

2019-01-25 | GSE125572 | GEO

microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

Project description:microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course

| PRJNA516857 | ENA

Loss of Elp3 impairs the immune response to helminths by blocking intestinal tuft cell differentiation

Project description:Intestinal tuft cells are critical in anti-helminth immunity by producing IL-25, which triggers IL-13 secretion by activated group 2 innate lymphoid cells (ILC2s) in order to ultimately expand both goblet and tuft cells. Translational reprogramming is involved in intestinal tuft cell differentiation but the role of tRNA modifications in this process is unknown. We show here that epithelial Elp3, a tRNA-modifying enzyme, promotes tuft cell differentiation and is consequently critical for IL-25 production, ILC2 activation, goblet cell expansion and control of N. brasiliensis infection in mice. Elp3 is essential for the IL-13-dependent induction of some glycolytic enzymes such as Hexokinase 1 and Aldolase A and consequently links specific metabolic pathways to tuft cell differentiation. Importantly, loss of epithelial Elp3 in the intestine blocks the translation of Nprl2, a mTORC1 inhibitor, which consequently enhances mTORC1 activation and stabilizes Atf4 in both transit amplifying and progenitor cells. Likewise, Atf4 overexpression in mouse intestinal epithelium blocks tuft cell differentiation and impairs the control of intestinal helminth infection. Collectively, our data define Atf4 as a negative regulator of tuft cell differentiation and provide insights into mechanisms through which some tRNA modifications promote a type 2 immune response to parasites in the intestine.

2024-07-25 | GSE241625 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data