Project description:To investigate the potential effect of grazing movement on miRNA circulation in cattle, here we profiled miRNA expression in centrifugally prepared exosomes from the plasma of both grazing and housed Japanese Shorthorn cattle. Microarray analysis of the c-miRNAs resulted in detection of a total of 231 bovine exosomal miRNAs in the plasma, with a constant expression level of let-7g across the duration and cattle groups. Expression of muscle-specific miRNAs such as miR-1, miR-133a, miR-206, miR-208a/b, and miR-499 were undetectable, suggesting the mildness of grazing movement as exercise.
Project description:We aimed to elucidate the effects of feeding condition (indoor grain-feeding vs. grazing on pasture) on c-miRNAs in Japanese Black (JB) cattle (Wagyu). The cattle at 18 months old were divided into pasture feeding and conventional indoor grain feeding for 5 months. Microarray analysis of c-miRNAs from the plasma extracellular vesicles led to the detection of a total of 202 bovine miRNAs in the plasma, including 15 miRNAs that differed between the feeding conditions.
Project description:RNA-Seq has been an effective method for exploring pathology-associated transcriptional changes in peripheral blood. The main goal of this study is to explore the expression changes of transcripts in the peripheral blood of sick pigs which may be infected with multiple pathogens on farms. We aim to find a subset of marker genes which may be useful candidate genes in the evaluation and prediction of health status in pigs under commercial production conditions.
Project description:Bovine mastitis is the most common disease that affects dairy cattle worldwide and generates millions of losses for cattle breeders. One of the most common pathogens identified in infected milk samples is Staphylococcus aureus. Presently, there is no fast, bacterial species-recognizing test in the diagnostic market.
The aim of this study was bioinformatic detection and characteristic of fibronectin binding protein A (FnBPA) of Staphylococcus aureus (SA) and investigation of this protein in milk samples obtained from cows with diagnosed mastitis.
Bioinformatic detection of potential biomarker for bovine SA obtained analyses of >90.000.000 amino acid sequences. Analyses on FnBPA included, among others: detection of signal peptides, non-classical proteins, antigenicity, and epitopes prediction. To confirm a presence of the fnbA gene in four SA isolates amplification with specific primers was performed. Detection of FnBPA was carried out by immunoblotting. Proteins mixture was separated by SDS-PAGE, and immunoreactivity and selectivity were performed by monoclonal anti-FnBPA antibodies and SA-negative serum.
Bioinformatic analyses showed that FnBPA is a surface, conservative, immunoreactive, and species-specific protein with antigenic potential. Molecular methods confirmed its presence in 100% of isolates, and immunoblotting proved its immunoreactivity and specificity. Thus, it can be considered as potential biomarker in immunodiagnostic of mastitis.
2023-01-26 | MSV000091181 | MassIVE
Project description:Analysis of Microbial Aerosols Diversity in Cattle Farms
| PRJNA1239932 | ENA
Project description:Nanopore detection of conifer seedborne pathogens
| PRJNA1226600 | ENA
Project description:Metagenomic Detection of Oomycete Plant Pathogens