Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The role of ESX-1 on infected and uninfected neutrophils in Mycobacterium marinum infection

ABSTRACT: The role of ESX-1 on infected and uninfected neutrophils in Mycobacterium marinum infection

PROVIDER: PRJNA721954 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14238739_1.fastq.gz	Fastqsanger.gz
	SRR14238739_2.fastq.gz	Fastqsanger.gz
	SRR14238740_1.fastq.gz	Fastqsanger.gz
	SRR14238740_2.fastq.gz	Fastqsanger.gz
	SRR14238741_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 96

Similar Datasets

The role of ESX-1 on infected and uninfected neutrophils in Mycobacterium marinum infection

Project description:Mice were infected with wild type or ESX-1-deficient (deltaRD1) Mycobacterium marinum. 14 days post infection, infected and uninfected neutrophils from the infected tissue were sorted for single cell RNA sequencing analysis.

2023-03-24 | GSE172072 | GEO

single cell RNA sequencing analysis of infected or bystander (noninfected) monocytes during M. marinum infection

Project description:Mice were infected with wild type, EspK::tn, or deltaRD1 (completely ESX-1-deficient) Mycobacterium marinum. 14 days post infection, infected and uninfected monocytes from the infected tissue were sorted for single cell RNA sequencing analysis.

2025-02-12 | GSE235124 | GEO

The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 sub-strates in Mycobacterium marinum

Project description:Raw and processed files in support of manuscript The loss of the PDIM/PGL virulence lipids causes differential secretion of ESX-1 sub-strates in Mycobacterium marinum The Virulence Lipid PDIM/PGL is essential for optimal protein secretion in Mycobacte-rium marinum WT, PDIM and complement strains lysate and culture filtrate analysis of proteomes

2023-12-21 | MSV000093713 | MassIVE

miR-126 knockdown and Mycobacterium marinum infection

Project description:We identify a putative link between miR-126 knockdown and neuroactive ligand and notch signalling pathways in uninfected and Mycobacterium marinum infected zebrafish embryos.

2024-01-23 | GSE253537 | GEO

Zebrafish response to Mycobacterium marinum infection (Affymetrix series)

Project description:Mycobacteria infect macrophages that aggregate with additional macrophages and lymphocytes to form granulomas. We have used a functional genomics approach to identify immune response genes expressed during granuloma formation in Mycobacterium marinum-infected transparent zebrafish larvae where individual infection steps can be viewed in real time. We assessed RNA expression profiles from zebrafish larvae that were either infected with Mycobacterium marinum, mock-infected, or uninfected. Zebrafish infections were performed at 1 day post-fertilization (dpf), and samples were derived from pools of 6dpf zebrafish larvae. Keywords: host response to infection

2008-01-01 | GSE8327 | GEO

Neutrophils infected with Leishmania donovani

Project description:In the context of studying visceral leishmaniasis, neutrophils infected with Leishmania donovani have been compared to uninfected neutrophils. Compared time points are 0, 6 and 24 hours post infection. Neutrophils of three human donors have been used. Overall 6 samples for infected neutrophils at time point 6 hours and 6 samples for infected neutrophils at time point 24 hours exist, including three biological samples and two technical samples. Uninfected neutrophils represent 3 samples at time point 0 hours, 3 samples at time point 6 hours and 3 samples at time point 24 hours. Transcriptome of Leishmania donovani culture has been assessed in two replicates.

| EGAS00001004912 | EGA

RNA seq analysis from the kidney neutrophils of pycard mutant adult zebrafish in Mycobacterium marinum infection

Project description:Inflammasome regulates the host response to intracellular pathogens including mycobacteria. We have previously shown that the course of M. marinum infection in adult zebrafish (Danio rerio) has similar features than the course of tuberculosis in human. Here we have investigated the role of the inflammasome adaptor pycard in M. marinum infection in zebrafish with two CRISPR/Cas9 mutagenesis generated zebrafish lines. Adult zebrafish devoid of pycard showed impaired survival and increased bacterial burden in M. marinum infection. Transcriptome analysis with RNA sequencing of a kidney derived neutrophils of M. marinum infected fish homozygous for pycardptu5 mutation revealed differentially expressed genes compared to WT controls. Genes associated with neutrophil degranulation, haematopoiesis and PI3K signalling are differentially expressed in the pycard deficient neutrophils when compared to wild type controls.

2025-02-07 | GSE287594 | GEO

Novel compounds modulating Mycobacterium marinum ESX-5 secretion display anti-mycobacterial activity in vivo

Project description:We observed through Western blot that Mycobacterium marinum secreted more highly abundant PE-PGRS proteins that are above 130 kDa when treated with compound 36 than mock-treated bacteria. We would like to explore which PE-PGRS proteins are more secreted in this condition and whether other proteins are also differentially secreted between two conditions.

2023-01-19 | PXD037981 | Pride

RNA seq analysis from the kidney neutrophils of pycard mutant adult zebrafish in Mycobacterium marinum infection

Project description:Inflammasome regulates the host response to intracellular pathogens including mycobacteria. We have previously shown that the course of M. marinum infection in adult zebrafish (Danio rerio) has similar features than the course of tuberculosis in human. Here we have investigated the role of the inflammasome adaptor pycard in M. marinum infection in zebrafish with two CRISPR/Cas9 mutagenesis generated zebrafish line. Adult zebrafish devoid of pycard showed impaired survival and increased bacterial burden in M. marinum infection. Transcriptome analysis with RNA sequencing of a kidney derived neutrophils of M. marinum infected fish homozygous for pycardptu4 mutation revealed differentially expressed genes compared to WT controls. Differentially expressed genes were associated with neutrophil degranulation, haematopoiesis and PI3K signalling are differentially expressed in the pycard deficient neutrophils when compared to wild type controls.

2024-07-03 | GSE270136 | GEO

EspM regulates gene expression in response to the ESX-1 system in Mycobacterium marinum

Project description:EspM regulates gene expression in response to the ESX-1 system in Mycobacterium marinum

| PRJNA557262 | ENA