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A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [Timecourse]

ABSTRACT: A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [Timecourse]

PROVIDER: PRJNA724678 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14309980.fastq.gz	Fastqsanger.gz
	SRR14309981.fastq.gz	Fastqsanger.gz
	SRR14309982.fastq.gz	Fastqsanger.gz
	SRR14309983.fastq.gz	Fastqsanger.gz
	SRR14309984.fastq.gz	Fastqsanger.gz

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A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [Timecourse]

Project description:Production of cultured meat requires the robust differentiation of satellite cells into mature muscle fibers without the use of animal-derived components. Current protocols induce myogenic differentiation in vitro through serum starvation, an abrupt reduction in serum concentration. Here, we used RNA sequencing to investigate the transcriptomic remodelling of bovine satellite cells during myogenic differentiation induced by serum starvation. We characterized canonical myogenic gene expression, and identified surface receptors upregulated during the early phase of differentiation. Supplementation of ligands to these receptors enabled the formulation of a chemically defined media that induced differentiation in the absence of serum starvation and/or transgene expression. Serum-free myogenic differentiation was of similar extent to that induced by serum starvation, as evaluated by transcriptome analysis, protein expression and the presence of a functional contractile apparatus. Moreover, the serum-free differentiation media supported the fabrication of mature three-dimensional bioartificial muscle constructs, demonstrating its suitability for cultured beef production.

2021-10-22 | GSE173198 | GEO

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [SF_Diff]

2021-10-22 | GSE173196 | GEO

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation

Project description:A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation

| PRJNA724671 | ENA

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [SF_Diff]

Project description:A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation [SF_Diff]

| PRJNA724677 | ENA

A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in the absence of serum starvation

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-10-22 | GSE173199 | GEO

Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium

Project description:The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which was then tested extensively. In this study, we present the properties of keratinocyte cultivated with serum-Free medium (Surge SFM) and complemented DME-Ham medium (with serum).

2021-09-08 | GSE183594 | GEO

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Project description:Activin A is known to induce endodermal differentiation in human embryonic stem cells under low-serum or certain defined media conditions. To gain insights into global gene expression changes during Activin A-induced differentiation, gene expression was monitored by means of microarrays in short intervals until 4 days of treatment. Keywords: Differentiation timecourse experiment

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