Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Growth media conditions influence the secretion route and release levels of engineered extracellular vesicles

ABSTRACT: Growth media conditions influence the secretion route and release levels of engineered extracellular vesicles

PROVIDER: PRJNA725794 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14341180.fastq.gz	Fastqsanger.gz
	SRR14341181.fastq.gz	Fastqsanger.gz
	SRR14341182.fastq.gz	Fastqsanger.gz
	SRR14341183.fastq.gz	Fastqsanger.gz
	SRR14341184.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Growth media conditions influence the secretion route and release levels of engineered extracellular vesicles

Project description:Extracellular vesicles (EVs) are nanosized cell-derived vesicles found in all bodily fluids which provide a route of intercellular communication by transmitting biological cargo. While EVs offer promise as therapeutic agents, the molecular mechanisms of EV biogenesis are not yet fully elucidated, in part due to the concurrence of numerous interwoven pathways which give rise to heterogenous EV populations in vitro. The effects of conditions in the cellular environment on EV production are of particular interest. In this study, we utilize a quantifiable EV-engineering approach to investigate how various cell media conditions alter EV production. The presence of serum, exogenous EVs, and other signaling factors in cell media alters EV production on physical, molecular, and transcriptional levels. Further, we demonstrate that exosome biogenesis pathways are the major factors contributing to EV production under optimized conditions. Our findings suggest a novel understanding to the mechanisms underlying EV production in cell culture which can be applied to develop advanced EV production methods.

2021-04-29 | GSE173474 | GEO

Growth Media Conditions Influence the Secretion Route and Release Levels of Engineered Extracellular Vesicles.

Project description:Extracellular vesicles (EVs) are nanosized cell-derived vesicles produced by all cells, which provide a route of intercellular communication by transmitting biological cargo. While EVs offer promise as therapeutic agents, the molecular mechanisms of EV biogenesis are not yet fully elucidated, in part due to the concurrence of numerous interwoven pathways which give rise to heterogenous EV populations in vitro. The equilibrium between the EV-producing pathways is heavily influenced by factors in the extracellular environment, in such a way that can be taken advantage of to boost production of engineered EVs. In this study, a quantifiable EV-engineering approach is used to investigate how different cell media conditions alter EV production. The presence of serum, exogenous EVs, and other signaling factors in cell media alters EV production at the physical, molecular, and transcriptional levels. Further, it is demonstrated that the ceramide-dependent EV biogenesis route is the major pathway to production of engineered EVs during optimized EV-production. These findings suggest a novel understanding to the mechanisms underlying EV production in cell culture which can be applied to develop advanced EV production methods.

| S-EPMC11469210 | biostudies-literature

Murine blastocysts release mature microRNAs into culture media that reflect developmental status

Project description:Murine blastocysts release mature microRNAs into culture media that reflect developmental status

| PRJNA575279 | ENA

Immunoglobulin-like domain receptor 1 mediates fat-stimulated cholecystokinin secretion.

Project description:Cholecystokinin (CCK) is a satiety hormone produced by discrete enteroendocrine cells scattered among absorptive cells of the small intestine. CCK is released into blood following a meal; however, the mechanisms inducing hormone secretion are largely unknown. Ingested fat is the major stimulant of CCK secretion. We recently identified a novel member of the lipoprotein remnant receptor family known as immunoglobulin-like domain containing receptor 1 (ILDR1) in intestinal CCK cells and postulated that this receptor conveyed the signal for fat-stimulated CCK secretion. In the intestine, ILDR1 is expressed exclusively in CCK cells. Orogastric administration of fatty acids elevated blood levels of CCK in wild type but not ILDR1-deficient mice, although the CCK secretory response to trypsin inhibitor was retained. The uptake of fluorescently labeled lipoproteins in ILDR1-transfected CHO cells and release of CCK from isolated intestinal cells required a unique combination of fatty acid plus HDL. CCK secretion secondary to ILDR1 activation is associated with increased [Ca2+]i consistent with regulated hormone release. These findings demonstrate that ILDR1 regulates CCK release through a mechanism dependent on fatty acids and lipoproteins and that absorbed fatty acids regulate gastrointestinal hormone secretion. GFP positive cells from CCK-EGFP transgenic mice were isolated by FACS and the expression profile was compared with an equal number of non-fluorescent intestinal cells.

2013-05-23 | E-GEOD-47196 | biostudies-arrayexpress

Route specific dermal responses to vaccinia virus infection

Project description:Transcriptional profiles of rabbit skin infected via the intradermal or scarification route of infeciton assessing the early time post infection. Transcriptional profiles were assessed at 4hrs, 24hrs, and 48hrs post infection for each route of infection. Transcript variation is as compared to normal, uninfected rabbit skin.

2014-05-13 | GSE57564 | GEO

Route specific dermal responses to vaccinia virus infection

2014-05-13 | E-GEOD-57564 | biostudies-arrayexpress

Murine blastocysts release mature microRNAs into culture media that reflect developmental status

Project description:Extracellular microRNA sequences have attracted interest for their potential use as accessible biomarkers that may be non-invasively collected from a wide range of biological fluids, including spent culture media. The ongoing milieu between the late-stage preimplantation embryo and the receptive uterus remains an actively investigated area of research and the physiological role of secreted microRNA sequences remains to be determined. Spent culture media used to culture late stage E4.0 murine blastocysts was screened for 641 mature microRNA sequences using a qPCR-based array. We report here the 39 sequences that were exclusively detected in the conditioned media using this approach. The embryonic miR-290 family were among the most abundant extracellular sequences. Notably, implantation-relevant members miR-126a, miR-101a, miR-143 and miR-320 were also detected.

2021-01-16 | GSE138285 | GEO

Plagued Silk Route

Project description:The Plagued Silk Route: Yersinia pestis molecular signatures in 17th century camelids from Romania

| PRJEB81205 | ENA

Plagued Silk Route

Project description:Plagued Silk Route: Yersinia pestis molecular signatures in a 17 th century camelid, Romania.

| PRJEB81137 | ENA

Chloropsis media

Project description:Chloropsis media

| PRJNA920886 | ENA