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Comparative transcriptomes of WT or jasmonic acid oxidase 2-1 (jao2-1) rosette leaves before or after inoculation with Botrytis cinerea spores

ABSTRACT: Comparative transcriptomes of WT or jasmonic acid oxidase 2-1 (jao2-1) rosette leaves before or after inoculation with Botrytis cinerea spores

PROVIDER: PRJNA740181 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14887000.fastq.gz	Fastqsanger.gz
	SRR14887001.fastq.gz	Fastqsanger.gz
	SRR14887002.fastq.gz	Fastqsanger.gz
	SRR14887003.fastq.gz	Fastqsanger.gz
	SRR14887004.fastq.gz	Fastqsanger.gz

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Comparative transcriptomes of WT or jasmonic acid oxidase 2-1 (jao2-1) rosette leaves before or after inoculation with Botrytis cinerea spores

Project description:The deficiency of Jasmonic Acid Oxidase 2 (JAO2) disrupts a metabolic sink in jasmonate hormonal pathway. Consequently, higher flux towards hormone JA-Ile is observed and some leaf JA responses have elevated basal levels relative to wild-type (WT) leaves. We report the compared transcriptomes of WT and jao2-1 rosette leaves, before and 48h after inoculation with spores of the fungus Botrytis cinerea. The study reveals the set of jao2-deregulated genes before infection

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Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. We identified by ChIP-seq >1680 high-confidence WRKY33 binding sites associated with 1576 genes within the Arabidopsis genome, with all of them being dependent on rapid activation of WRKY33 expression by Botrytis cinerea strain 2100. Genome-wide transcriptional analysis defined 318 genes as direct functional targets at 14 h post inoculation. Comparison between resistant wild-type Columbia-0 and susceptible wrky33 mutant plants revealed that expression of 75% of all WRKY33 regulated targets were down-regulated upon infection, indicating that WRKY33 predominately acts as a repressor. However, WRKY33 appears to possess dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. Our genome-wide analysis confirmed known WRKY33 targets involved in ethylene and jasmonic acid hormone signaling and phytoalexin biosynthesis, but also uncovered a previously unknown role of abscisic acid (ABA) biosynthesis in the complex regulatory circuitry affecting resistance towards Botrytis. Analysis of transgenic plants expressing WRKY33-HA under its native promoter post inoculation with spores of Botrytis cinerea 2100

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