Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.
Project description:The emergence of triazole resistance in Aspergillus fumigatus poses a significant threat to clinical antifungal therapy. This study investigated the impact of prolonged exposure to the agricultural triazole tebuconazole (TEB) on the susceptibility of A. fumigatus to clinical triazoles and explored associated resistance mechanisms. After 28 days of exposure, isolates exhibited substantial increases in minimum inhibitory concentrations (MICs): up to 128-fold for itraconazole, 64-fold for posaconazole, and 16-fold for voriconazole. Resistance persisted even after TEB removal. Ergosterol levels remained stable, while efflux pump inhibition led to MIC reduction, confirming their role in resistance. Proteomic analysis identified 193 upregulated and 88 downregulated proteins, with distinct profiles between treated and untreated groups. These findings demonstrate that agricultural triazoles can induce stable, multidrug-resistant phenotypes in A. fumigatus, emphasizing the need for integrated surveillance of antifungal resistance across environmental and clinical settings.
Project description:This SuperSeries is composed of the following subset Series: GSE24983: Response of A549 cells treated with Aspergillus fumigatus [WT-CF_vs_WT-GC] GSE24984: Response of A549 cells treated with Aspergillus fumigatus [WT-GC_vs_PrtT-GC] GSE24985: Response of A549 cells treated with Aspergillus fumigatus [WT-CF_vs_PrtT-CF] Refer to individual Series
Project description:Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the small RNA repertoire of A. fumigatus in conidia and mycelium grown for 24 or 48 hours in liquid culture.
Project description:Experimantal validation for the 5' and 3' ends of poly(A)-RNA in Aspergillus fumigatus is currently lacking. The dataset here was used a total RNA-Seq control for poly(A)-enriched RNAs of Aspergillus fumigatus in comparison with 5' and 3' End-Seq data.
Project description:Amphotericin B (AMB) is the most widely used polyene antifungal drug for the treatment of systemic fungal infections including invasive aspergillosis. We aimed to understand molecular targets of AMB in Aspergillus fumigatus (Afu) by genomic approaches. Keywords: Aspergillus fumigatus treated with amphotericin B for 24 hours
