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Characterizing the molecular-physiological basis of pollen abortion upon prolonged high temperature stress

ABSTRACT: Characterizing the molecular-physiological basis of pollen abortion upon prolonged high temperature stress

PROVIDER: PRJNA770197 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16288656_1.fastq.gz	Fastqsanger.gz
	SRR16288656_2.fastq.gz	Fastqsanger.gz
	SRR16288657_1.fastq.gz	Fastqsanger.gz
	SRR16288657_2.fastq.gz	Fastqsanger.gz
	SRR16288658_1.fastq.gz	Fastqsanger.gz

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Characterising the molecular-physiological basis of pollen abortion upon prolonged high temperature stress.

Project description:Characterising the molecular-physiological basis of pollen abortion upon prolonged high temperature stress.

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Characterising the molecular-physiological basis of pollen abortion upon prolonged high temperature stress.

Project description:We compared the transcriptomic response of polarized microspore stage tomato anthers to long-term mild heat (LTMH-) stress of wild-type and three lines that display increased pollen thermo-tolerance. Our results indicated distinct differences between the thermo-tolerant lines and wild-type, suggesting a dampened response to LTMH in the tolerant lines than in wild-type.

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Characterizing the molecular-physiological basis of pollen abortion upon prolonged high temperature stress

Project description:We evaluated the transcriptomic response of developing tomato anthers to long-term mild heat (LTMH-) stress. Our results indicated changes in HSR-, carbohydrate-, and phytohormone-related gene expression and a delayed reduction in tapetum-related gene expression.

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wheat, pollen, MALDI-TOF/TOF-MSMS

Project description:In order to better understand chemical hybridizing agent (CHA) SQ-1-induced pollen abortion in wheat better, comparative proteomic analyses were conducted. A total of 61 proteins showed statistically significant differences in abundance, among which, 18 proteins were highly abundant and 43 proteins were less abundant in CHA-SQ-1 treated plants. Here, 60 proteins were successfully identified using MALDI-TOF/TOF mass spectrometry. These proteins were found to be involved in pollen maturation. Interactions between these proteins were predicted using bioinformatics analysis. Gene ontology and pathway analyses revealed that the majority of the identified proteins were involved in diverse biological processes. These results provide information for the molecular events (carbohydrate metabolism and energy metabolism) underlying CHA-SQ-1-induced pollen abortion and may serve as an additional guide for practical hybrid breeding.

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A RALF-like peptide EaF82 derived from Epipremnum aureum inhibits pollen development in Arabidopsis and tobacco

Project description:To investigate the function of previously cloned novel cysteine-rich peptide EaF82 which biochemical properties are similar to the Rapid Alkalinization Factor (RALF), we overexpressed EaF82 gene in Arabidopsis and found defects only in the pollen development. To further determine the affected genes and biological pathways, we compared the transcriptome profilings of two independent Arabidopsis EaF82 overexpressing lines (TA and TB) with that of the vector control line (C). The RNAseq analysis results revealed decreased expressions of five endogenous clade IV AtRALFs and genes responsible for cell wall modifications and pollen maturation, supporting the observed pollen abortion.

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Cytological and transcriptome analysis at meiosis and mitosis reveals insights into pollen fertility in synthetic Brassica allohaploid by crosses between B. nigra and B. rapa

Project description:Purpose:The molecular mechanism of pollen abortion in interspecific hybrids of B.rapa and B.nigra was revealed by cytological and transcriptional analysis Methods:floral buds (contain two developmental progress,1.1-1.5 mm and 1.5-6.0 long floral buds) were collected, then Separated male organs were kept in liquid nitrogen immediately until use. Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). DNase (Promega, USA) was used to remove potential DNA contamination. For the quantitative real-time polymerase chain reaction (qRT-PCR) analysis Results:In this study, there were extensive variations in gene expression patterns of this hybrid, and down-regulated expression of key genes for pollen development, such as meiosis of pollen mother cells, DNA damage repair and pollen sperm cell differentiation, may lead to pollen abortion. Styrene-propyl synthesis and wax biosynthesis pathway were blocked, which affected pollen wall formation. Conclusions:The wide variation of genes showed that the expression of A and B genomes were affected differently. B genome tended to downregulate the expression of key genes during meiosis and mitosis, while A genome tended to downregulate the expression of genes related to stress and pollen wall formation. The results showed that the development of male gametophyte was seriously affected after synthesis.

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Heat stress induced proteomes in tomato pollen mother cells and microspores

Project description:During the reproductive stage, tomatoes require nighttime temperatures below 21°C and daytime temperatures below 32°C for healthy flower development and fruit set. Prolonged exposure to temperatures above the optimal range can cause abnormalities in pollen development, leading to reduced fertility or sterility. This project aims to identify alterations in proteome expression and elucidate heat tolerance mechanisms affecting pollen development under elevated temperature conditions. Two tomato varieties—the heat-tolerant ‘Black Vernissage’ and the heat-sensitive ‘Micro-Tom’—were exposed to three temperature treatments: 26 °C (control), 32 °C, and 37 °C (heat-treated). Flower buds containing meiotic pollen mother cells prior to the tetrad stage and free microspores were harvested, and single cell type samples were collected using laser microdissection. Tandem mass tag (TMT) proteomics was employed to identify differential proteomic responses in pollen mother cells and free microspores between stress-treated and non-treated plants.

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