Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone or dexamethasone+cycloheximide treatment. Tissue samples were collected at 3hrs after the treatment. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (Agilent, custom-commercial).
Project description:Protein interaction partners of the exon 5,6-deleted AP1 mutant fused to GFP in the ap1-11 mutant background (AP1tet-GFP ap1-11), the site-directed mutagenized L154P and L168P AP1 mutant fused to GFP in the ap1-11 mutant background (AP1dm-GFP ap1-11), and the wild type AP1 fused to GFP in the ap1-11 mutant background (AP1-GFP ap1-11) were studied by GFP immunoprecipitation followed by mass spectrometry (IP-MS).
