Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a 35S:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone or dexamethasone+cycloheximide treatment. Tissue samples were collected at 3hrs after the treatment. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (Agilent, custom-commercial).
Project description:Protein interaction partners of the exon 5,6-deleted AP1 mutant fused to GFP in the ap1-11 mutant background (AP1tet-GFP ap1-11), the site-directed mutagenized L154P and L168P AP1 mutant fused to GFP in the ap1-11 mutant background (AP1dm-GFP ap1-11), and the wild type AP1 fused to GFP in the ap1-11 mutant background (AP1-GFP ap1-11) were studied by GFP immunoprecipitation followed by mass spectrometry (IP-MS).
Project description:This experiment describes gene expression after the activation of APETALA1-GR, to study and identify AP1 target genes. We used a pAP1:AP1-GR ap1 cal line to induce a synchronized response activating the AP1-GR fusion protein in ap1 cal inflorescence-like meristems through dexamethasone treatment. Tissue samples were collected immediately after the treatment, as well as subsequent timepoints. The expression profiles of the individual samples were then analyzed by gene expression profiling using whole-genome oligonucleotide arrays (Agilent, custom-commercial)
