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Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and pkl seedlings.

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and pkl seedlings.

PROVIDER: PRJNA772746 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16484600_1.fastq.gz	Fastqsanger.gz
	SRR16484600_2.fastq.gz	Fastqsanger.gz
	SRR16484601_1.fastq.gz	Fastqsanger.gz
	SRR16484601_2.fastq.gz	Fastqsanger.gz
	SRR16484596_1.fastq.gz	Fastqsanger.gz

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Identify differentially expressed genes in 14-day-old pkl seedlings

Project description:CHD3 proteins are ATP-dependent chromatin remodeling factors that are components of diverse multisubunit complexes that can either repress or activate gene expression. In plants, the CHD3 protein PICKLE (PKL) is necessary for repression of seed-specific genes during germination and promotes deposition of the repressive epigenetic mark trimethylation of histone H3 lysine 27 (H3K27me3). It is unknown, however, if PKL acts directly at H3K27me3-enriched loci. We undertook a microarray analysis of 14-day-old plants and found that PKL continues to play an important role in expression of H3K27me3-enriched genes and in specification of developmental identity after germination. We used microarray to identify genes that are differentialy expressed in 14-day-old pkl seedlings and used chormatin immunoprecipitation to identify genes that are the direct targets of PKL.

2012-01-01 | GSE31639 | GEO

Identify differentially expressed genes in 14-day-old pkl seedlings

2012-01-01 | E-GEOD-31639 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and pkl seedlings.

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: RNA from 14-day-old seedlings of wild-type and pkl was isolated using the RNAprep Pure Plant Kit as described above. RNAs from three biological replicates was sequenced separately at Novogene, using Illumina Hiseq X-Ten (Sequencing method: Hiseq-PE150). Results: Reads were mapped to the TAIR10 Arabidopsis genome using TopHat (Galaxy V2.1.1 in usegalaxy.org) with default settings, except that a minimum intron length of 20 bp and a maximum intron length of 5,000 bp were required (Paired-end). Then, mapped reads were assembled according to TAIR10 version of genome annotation using cufflinks (version 2.1.1) with default settings. To analyze differential expression, the assembled transcripts from three independent biological replicates in Col and other mutants were included and compared using Cuffdiff (version 2.1.1) with default settings. 14-day-old seedling of WT and pkl were performed RNA-seq to analyzed their differential expression genes in Arabidopsis.

2022-12-12 | GSE186152 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and val1 val2 pkl seedlings.

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: RNA from 14-day-old seedlings of wild-type and pkl was isolated using the RNAprep Pure Plant Kit as described above. RNAs from three biological replicates was sequenced separately at Novogene, using Illumina Hiseq X-Ten (Sequencing method: Hiseq-PE150). Results: Reads were mapped to the TAIR10 Arabidopsis genome using TopHat (Galaxy V2.1.1 in usegalaxy.org) with default settings, except that a minimum intron length of 20 bp and a maximum intron length of 5,000 bp were required (Paired-end). Then, mapped reads were assembled according to TAIR10 version of genome annotation using cufflinks (version 2.1.1) with default settings. To analyze differential expression, the assembled transcripts from three independent biological replicates in Col and other mutants were included and compared using Cuffdiff (version 2.1.1) with default settings. 14-day-old seedling of WT and val1 val2 pkl were performed RNA-seq to analyzed their differential expression genes in Arabidopsis.

2022-12-12 | GSE186156 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and val1 val2 pkl seedlings.

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt and val1 val2 pkl seedlings.

| PRJNA772750 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt, syd-5 and swi3d-1 seedlings [RNA-seq]

Project description:To investigate the cooperative function swi3d-1 and syd-5 in regulating plant development. We then performed gene expression profiling analysis using data obtained from RNA-seq of 14-day-old wt, syd-5 and swi3d-1 seedlings under long day condition.

2023-04-24 | GSE218842 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt, brm-1, brd1,2,13 and brm-1 brd1,2,13 seedlings

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Methods: RNA from 14-day-old seedlings of wild-type, brip1, brip2, brm-3, brm-1, brip1 brip2, brip1 brip2 brm-3 and brip1 brip2 brm-1 was isolated using the RNAprep Pure Plant Kit as described above. RNAs from three biological replicates was sequenced separately at Novogene, using Illumina Hiseq X-Ten (Sequencing method: Hiseq-PE150). Results: Reads were mapped to the TAIR10 Arabidopsis genome using TopHat (Galaxy V2.1.1 in usegalaxy.org) with default settings, except that a minimum intron length of 20 bp and a maximum intron length of 4,000 bp were required (Paired-end). Then, mapped reads were assembled according to TAIR10 version of genome annotation using cufflinks (version 2.1.1) with default settings. To analyze differential expression, the assembled transcripts from three independent biological replicates in Col and other mutants were included and compared using Cuffdiff (version 2.1.1) with default settings. Conclusions: Our study represents the first detailed analysis of brip1, brip2, brip1 brip2 transcriptomes, with biologic replicates, generated by RNA-seq technology.

2021-03-22 | GSE161516 | GEO

Expression data from aerial parts of 14-day-old wild-type and GFP-TIE1-15 Arabidopsis seedlings

Project description:TIE1 regulates leaf development by repressing leaf differentiation because the semi-dominant mutant tie1-D by activation tagging displays small and hyponastic leaves and the differentiation of leaf epidermal cells is delayed in the tie1-D mutant, whereas disruption of TIE1 causes epinastic leaves with early differentiated epidermal cells. We used microarrays to investigate the molecular base underpinning the phenotypes of TIE1-overexpressing plants. Aerial parts of 14-day-old seedlings from wild-type and GFP-TIE1-15 were collected for RNA extraction and hybridization on Affymetrix microarrays.

2012-05-26 | E-GEOD-38282 | biostudies-arrayexpress

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt, syd-5 and swi3d-1 seedlings [RNA-seq]

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt, syd-5 and swi3d-1 seedlings [RNA-seq]

| PRJNA905837 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt, brm-1, brd1,2,13 and brm-1 brd1,2,13 seedlings

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of in 14-day-old wt, brm-1, brd1,2,13 and brm-1 brd1,2,13 seedlings

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