Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Distinct translatome changes in specific neural populations precede electroencephalographic changes in prion-infected mice

ABSTRACT: Distinct translatome changes in specific neural populations precede electroencephalographic changes in prion-infected mice

PROVIDER: PRJNA783902 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR17047700.fastq.gz	Fastqsanger.gz
	SRR17047701.fastq.gz	Fastqsanger.gz
	SRR17047702.fastq.gz	Fastqsanger.gz
	SRR17047703.fastq.gz	Fastqsanger.gz
	SRR17047704.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 98

Similar Datasets

Distinct translatome changes in specific neural populations precede electroencephalographic changes in prion-infected mice

Project description:Selective vulnerability is an enigmatic feature of neurodegenerative diseases (NDs), whereby a widely expressed protein causes lesions in specific cell types and brain regions. Using the RiboTag method in mice, translational responses of five neural subtypes to acquired prion disease (PrD) were measured. Pre-onset and disease onset timepoints were chosen based on longitudinal electroencephalography (EEG) that revealed a gradual increase in theta power between 10- and 18-weeks after prion injection, resembling a clinical feature of human PrD. At disease onset, marked by significantly increased theta power and histopathological lesions, mice had pronounced translatome changes in all five cell types despite appearing normal. Remarkably, at a pre-onset stage, prior to EEG and neuropathological changes, we found that 1) translatomes of astrocytes indicated reduced synthesis of ribosomal and mitochondrial components, 2) glutamatergic neurons showed increased expression of cytoskeletal genes, and 3) GABAergic neurons revealed reduced expression of circadian rhythm genes. These data demonstrate that early translatome responses to neurodegeneration emerge prior to conventional markers of disease and are cell type-specific. Therapeutic strategies may need to target multiple pathways in specific populations of cells, early in disease.

2022-06-13 | GSE189527 | GEO

Distinct translatome changes in specific neural populations precede electroencephalographic changes in prion-infected mice.

| S-EPMC9401167 | biostudies-literature

Detection of genome-wide methylation changes in prion-infected central nervous system

Project description:Scrapie is a Transmissible Spongiform Encephalopathy (TSE) that affects sheep and goats and it is considered a good natural animal model to study prion diseases. Although changes in DNA methylation occur in many neurodegenerative diseases including human prion diseases, potential DNA methylation alterations have not yet been investigated in the central nervous system (CNS) of any prion disease models or naturally infected cases. We present here a whole genome bisulfite sequencing analysis (WGBS) from thalamus of four naturally scrapie infected sheep and four controls. All animals were female, carried the ARQ/ARQ genotype for the PRNP allele and were sacrificed at similar age (4 to 6 years old). Even if genomes displayed similar average methylation levels, we identified 39 differentially methylated promoters (DMP) and a total of 8,907 differentially methylated regions (DMR). Gene Ontology enrichment revealed that hypomethylated DMRs were enriched in genes involved in transmembrane transport and cell adhesion whereas hypermethylated DMRs were related with intracellular signal transduction genes. Moreover, we compared the set of identified DMRs with genes previously described to be differentially expressed in scrapie and with a set of genes encoding proteins defined to be tenfold more abundant in specific types of CNS cells finding that some of these genes also harboured DMRs. Finally, a validation study using qPCR was conducted showing differences in the expression of five genes (PCDH19, SNCG, WDR45B, PEX1 and CABIN1) that matched the methylation changes observed in the genomic study. Altogether, these findings suggest a potential regulatory role of CNS DNA methylation in prion diseases.

2022-03-10 | GSE184767 | GEO

Prion infected ScN2a and GT1

Project description:A comparison of prion infected and non-infected samples from neuroblastoma cells (N2a), and a comparison of prion infected and non-infected samples from hypothalmus cells (GT1). 11 dual-color DNA-chip hybridizations of cDNAs were made. Keywords: other

2005-08-24 | GSE1803 | GEO

Prion infected ScN2a and GT1

2010-06-09 | E-GEOD-1803 | biostudies-arrayexpress

Cell-type specific translational changes in prion diseases

Project description:Identification of genome-wide translational changes during prion disease via cell-type specific ribosome profiling.

2020-09-29 | GSE149805 | GEO

Cell-type specific translational changes in prion diseases

Project description:Cell-type specific translational changes in prion diseases

| PRJNA630198 | ENA

Efficacy and molecular mechanism of a glycoside compound inhibiting abnormal prion protein formation in prion-infected cells

Project description:This report describes our study of the efficacy and the potential mechanism underlying the anti-prion action of a new anti-prion compound having a glycoside structure in prion-infected cells. The study revealed involvements of two factors in the mechanism of the compound action: interferon and a microtubule nucleation activator, phosphodiesterase 4D interacting protein. In particular, phosphodiesterase 4D interacting protein was suggested to be important in regulating the trafficking or fusion of prion protein-containing vesicles or structures in cells. The findings of the study are expected to be useful not only for the elucidation of cellular regulatory mechanisms of prion protein, but also for the implication of new targets for therapeutic development.

2014-02-22 | GSE55241 | GEO

Changes in adenoviral chromatin organization precede early gene activation

Project description:Changes in adenoviral chromatin organization precede early gene activation

| PRJNA562677 | ENA

Early epigenetic changes precede genetic alterations and drive hepatocarcinogenesis

Project description:Early epigenetic changes precede genetic alterations and drive hepatocarcinogenesis

| PRJNA609989 | ENA