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Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration

ABSTRACT: Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration

PROVIDER: PRJNA786586 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17143952_1.fastq.gz	Fastqsanger.gz
	SRR17143952_2.fastq.gz	Fastqsanger.gz
	SRR17143953_1.fastq.gz	Fastqsanger.gz
	SRR17143953_2.fastq.gz	Fastqsanger.gz
	SRR17143954_1.fastq.gz	Fastqsanger.gz

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Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration

Project description:Primary sensory neurons with cell soma in dorsal root ganglia (DRG) project axons in both the peripheral nervous system and the spinal cord. Whereas the peripheral axon can regenerate after injury, the central axon cannot, providing an opportunity to identify the mechanisms that promote axon regeneration. Using this system, multiple intrinsic mechanisms operating in sensory neurons have been shown to promote axon regeneration. Peripheral sensory neurons also benefit from a supportive environment. In particular, macrophages play important roles in nerve regeneration by clearing debris and regulating Schwann cell function at the site of injury in the nerve. Nerve macrophages have been characterized at the molecular level and derive for the most part from infiltrating monocytes. However, the role and origin of macrophages in the DRG remains poorly understood. Following a 14-days treatment with a CSF1R inhibitor, less than 10% of macrophages remained in the DRG. Recovery from this treatment resulted in a dramatic macrophage repopulation, with the number of DRG macrophages reaching level similar to untreated mice three days post injury. These repopulated DRG macrophages contribute to promote axon regeneration. Our scRNA-seq analysis allow in-depth characterization of the repopulated DRG macrophages in response to nerve injury. These data will provide a better understanding of DRG macrophages and the molecular mechanisms by which they contribute to peripheral nerve repair. These studies may lead to new potential targets to promote axon regeneration.

2023-02-07 | GSE190283 | GEO

Gene expression from aged and young dorsal root ganglia after nerve injury

Project description:Sciatic nerve crush was performed on cohorts of 2-month and 24-month old animals. Resulting gene-expression data were generated from dorsal root ganglia 5 days after injury compared to naïve animals. Results show differences in intrinsic growth responses with normal aging. Total RNA taken from L4 and L5 dorsal root ganglia 5 days after injury 2-month and 24-month old animals at either day 0 or day 5 after sciatic nerve crush injury.

2014-07-30 | E-GEOD-58982 | biostudies-arrayexpress

Quantification of oxidized proteins in wild type and Nox2 deficient mouse dorsal root ganglia

Project description:Here we studied the NOX2 dependent redox-proteome in dorsal root ganglia in mice. The overall goal was to assess the degree of NOX2-dependent changes in oxidised proteins following exposure to enriched enviroment and sciatic nerve axotomy in dorsal root ganglia.

2020-10-13 | PXD018085 | Pride

Gene expression from aged and young dorsal root ganglia after nerve injury

2014-07-30 | GSE58982 | GEO

Dorsal root ganglia(DRG) proteomic analysis

Project description:Complete annotation mice dorsal root ganglia proteome

2023-05-09 | PXD041382 | Pride

A Transcriptomic Analysis of Neuropathic Pain in Rat Dorsal Root Ganglia Following Peripheral Nerve Injury

Project description:In this study, we screened the differentially expressed genes (DEGs) in the dorsal root ganglia (DRG) from rats with sham or partial sciatic nerve ligation (pSNL) surgery 7 days using RNAseq technique to explore the molecular mechanisms of neuropathic pain

2020-06-30 | GSE117526 | GEO

Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration.

Project description:Sensory neurons located in dorsal root ganglia (DRG) convey sensory information from peripheral tissue to the brain. After peripheral nerve injury, sensory neurons switch to a regenerative state to enable axon regeneration and functional recovery. This process is not cell autonomous and requires glial and immune cells. Macrophages in the DRG (DRGMacs) accumulate in response to nerve injury, but their origin and function remain unclear. Here, we mapped the fate and response of DRGMacs to nerve injury using macrophage depletion, fate-mapping, and single-cell transcriptomics. We identified three subtypes of DRGMacs after nerve injury in addition to a small population of circulating bone-marrow-derived precursors. Self-renewing macrophages, which proliferate from local resident macrophages, represent the largest population of DRGMacs. The other two subtypes include microglia-like cells and macrophage-like satellite glial cells (SGCs) (Imoonglia). We show that self-renewing DRGMacs contribute to promote axon regeneration. Using single-cell transcriptomics data and CellChat to simulate intercellular communication, we reveal that macrophages express the neuroprotective and glioprotective ligand prosaposin and communicate with SGCs via the prosaposin receptor GPR37L1. These data highlight that DRGMacs have the capacity to self-renew, similarly to microglia in the Central nervous system (CNS) and contribute to promote axon regeneration. These data also reveal the heterogeneity of DRGMacs and their potential neuro- and glioprotective roles, which may inform future therapeutic approaches to treat nerve injury.

| S-EPMC9963351 | biostudies-literature

Transcriptional profiling of BXD dorsal root ganglia

Project description:We sequenced dorsal root ganglia mRNA from 25 BXD recombinant inbred mouse strains to determine their variation in gene expression. Dorsal root ganglia mRNA profiles of recombinant inbred mouse strains

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Deep proteome profiling of dorsal root ganglia after nerve injury deciphers shared signatures in adolescent and adult male and female mice

Project description:Our understanding of how sex and age influence pathological pain at the molecular level is still limited. This is of high relevance for pediatric and adolescent patients, as they are known to be particularly vulnerable to long-term consequences of pathological pain. Here, we leveraged deep proteome profiling of mouse dorsal root ganglia (DRG) from the spared nerve injury (SNI)-model of neuropathic pain and investigated adolescent (4-week-old) and adult (12-week-old) male and female mice in parallel. Differential expression and multidimensional analysis enabled us to reveal sex- and age-dependent proteome regulation upon nerve injury. To enhance the translational significance of our findings, we determined shared proteome signatures among tested sex and age groups. By cross-referencing our results with human DRG data evolutionary conserved molecular patterns were identified. These not only bridge the gap between animal models and human biology, but also offer valuable insights for drug discovery efforts benefiting adolescents, women, and men equally. Overall, we provide an innovative resource that allows researchers to gain a more nuanced understanding of nerve injury-induced changes in mouse DRG. Our findings have significant implications for translational research, potentially accelerating discoveries in peripheral nervous system function and pain.

2024-12-19 | PXD055816 | Pride

Expression data from the dorsal root ganglia during streptozotocin-induced painful diabetic neuropathy in rats

Project description:FK1706 potentiated nerve growth factor-induced neurite outgrowth, putatively mediated via FKBP-52 and the Ras/Raf/MAPK signaling pathway. It also improved mechanical allodynia accompanied by the recovery of intraepidermal nerve fiber density in a painful diabetic neuropathy in rats. We analyzed gene expression of the dorsal root ganglia together with measurement of mechanical allodynia in diabetic rats to try to capture the global fingerprint of changes in gene expression associated with FK1706 administration and also to elucidate the putative mechanisms of its neurotrophic activity in vivo. Normal, STZ-treated controls, and STZ + FK1706 groups were used to evaluate gene expression analysis. Dorsal root ganglia from L4 to L6 were collected at the day after 1, 2, and 3 weeks of FK1706 administration, were immediately immersed in RNAlater reagent, and stored as instructed before RNA extraction. Equal amounts of total RNA from five rats were collected for each group.

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