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Single-cell Sequencing of Microglia following Repopulation Reveals Distinct Clusters and Transcriptomic Signatures in the 3xTg Alzheimer's Disease Model

ABSTRACT: Single-cell Sequencing of Microglia following Repopulation Reveals Distinct Clusters and Transcriptomic Signatures in the 3xTg Alzheimer's Disease Model

PROVIDER: PRJNA787636 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17189087.fastq.gz	Fastqsanger.gz
	SRR17189088.fastq.gz	Fastqsanger.gz
	SRR17189089.fastq.gz	Fastqsanger.gz
	SRR17189090.fastq.gz	Fastqsanger.gz
	SRR17189091.fastq.gz	Fastqsanger.gz

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Single-cell Sequencing of Microglia following Repopulation Reveals Distinct Clusters and Transcriptomic Signatures in the 3xTg Alzheimer's Disease Model

Project description:In this study, we aimed to deplete the aging microglia and investigate the impact of the newly-born microglia in Alzheimer's pathology using the 3xTg mouse model. Specifically, >24 mo male 3xTg mice were given chow containing 1200mg/kg PLX5622 (AIN-76A-D1001i, Research Diests, NJ, USA) for 2 weeks to deplete their microglia. Control diet with the same base formula were given to control group and to experimental groups during a 4-week repopulation phase immediately following PLX treatment. At the end of the repopulation, we pooled sorted, viable microglia (CD45int CD11b) from 3-5 mice/group and ran single-cell RNAseq analysis to determine the similarities and differences between the aged and repopulated cells in the context of Alzheimer's Disease. We also included non-transgenic controls as a separate group.

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