Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Collagen10-Cre Rosa26-tdTomato e16.5 single cell RNA-sequencing

ABSTRACT: Collagen10-Cre Rosa26-tdTomato e16.5 single cell RNA-sequencing

PROVIDER: PRJNA787704 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Collagen10-Cre;Rosa26-tdTomato e16.5 single cell RNA-sequencing

Project description:In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse total bone isolated at e16.5 by Collagenase II digestion after mechanical digestion and soft tissue removal. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined chondrocytes, skeletal stem and progenitor cells (SSPCs), and osteoblasts. Trajectory analysis reveals that the SSPCs lie intermediate to the transition of chondrocyte to osteoblast. We conclude that hypertrophic chondrocytes dedifferentiate to this progenitor stage before further differentiation.

2022-02-16 | GSE190616 | GEO

Collagen10-Cre Rosa26-tdTomato bone marrow single cell RNA-sequencing

Project description:Collagen10-Cre Rosa26-tdTomato bone marrow single cell RNA-sequencing

| PRJNA742416 | ENA

Collagen10-Cre;Rosa26-tdTomato bone marrow single cell RNA-sequencing

Project description:In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse bone marrow harvested at 2 months of age by centrifugation and light Collagenase II digestion. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined skeletal stem and progenitor cells as well as CXCL12 abundant reticular (CAR) cells. These cells appear to be upstream of both osteoblasts and adipocytes. We conclude that hypertrophic chondrocytes dedifferentiate to this progentior stage before further differentiation.

2022-02-16 | GSE179148 | GEO

Collagen10a1-Cre Rosa26-tdTomato Bone marrow colony forming units

Project description:Collagen10a1-Cre Rosa26-tdTomato Bone marrow colony forming units

| PRJNA742567 | ENA

Prx1Cre;Rosa26-tdTomato single cell RNA-sequencing of muscle resident non-myogenic mesenchymal cells

Project description:In order to assess the descendants of lateral plate mesoderm within the muscle interstitium, we utilized Prrx1Cre;Rosa26-tdTomato P21 tdTomato+ FACS sorted muscle interstitial cells

2022-05-09 | GSE200234 | GEO

MERFISH analysis of Sst-Cre mediated Dnmt1 knockout at E16.5

Project description:To investigate the effects of Dnmt1 Knockout in Sst expressing interneurons on celltype distribution in the embryonic brain, Sst-Cre/tdTomato/Dnmt1 loxp2 knockout mice and Sst-Cre/tdTomato control mice were analysed. 10 µm coronal sections of E16.5 brains were prepared for the Merscope platform using the commercially available Pan Neuro panel (Vizgen), covering 500 distinct transcripts.

2025-06-18 | GSE298591 | GEO

Prx1Cre Rosa26-tdTomato single cell RNA-sequencing of muscle resident non-myogenic mesenchymal cells

Project description:Prx1Cre Rosa26-tdTomato single cell RNA-sequencing of muscle resident non-myogenic mesenchymal cells

| PRJNA823713 | ENA

Gene expression profile at single cell level of tdTomato+ DRG cells from Nav1.8-Cre R26CAG-floxStop-tdTomato mice

Project description:Gene expression profile at single cell level of tdTomato+ DRG cells from Nav1.8-Cre R26CAG-floxStop-tdTomato mice

| PRJNA906104 | ENA

In vivo RNA-seq for for Hh Dependent genes in E7.5 mesoderm

Project description:Mesp1-Cre+ cells from E7.5 mouse embryos (from the cross Mesp1-Cre/+ x Rosa26-Gli3R-IRES-YFP/tdTomato) were sorted by FACS where wild type (tdTomato-expressing) and mutant (Gli3R + YFP co-expressing) cells were collected separately from single litters.

2020-05-28 | GSE147868 | GEO

Bulk RNA sequencing of endothelial-derived and non-endothelial-derived BMSCs and OLCs of VE-Cadherin-Cre;LoxP-tdTomato mice

Project description:We performed lineage tracing experiments using VE-Cadherin-Cre;LoxP-tdTomato mice. In these mice, endothelial cells (ECs) and their progeny are permanently marked by tdTomato fluorescence. We found that a substantial subset of stromal cells is derived from ECs, as indicated by their tdTomato expression. These findings support the notion that endothelial to mesenchymal transition (EndoMT) contributes to hematopoietic bone marrow niche formation in mice. Here we sought to determine the transcriptomic differences between endothelial-derived (tdTomato-positive) and non-endothelial-derived (tdTomato-negative) bone marrow stromal cells (BMSCs) and osteo/chondrolineage progenitor cells (OLCs). Murine niche populations were obtained from collagenased bone fraction of VE-Cadherin-Cre;LoxP-tdTomato mice at 3 weeks (n=2) or 11 weeks (n=2) of age. BMSCs (CD45-TER119-CD31-CD144-SCA-1+ CD51+ cells) and OLCs (CD45-TER119-CD31-CD144-Sca1-CD51+ cells) were FACS-purified and sequenced.

2021-01-25 | E-MTAB-10020 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data