Project description:The combined activation of KrasG12D and loss of Cdkn2a leads to aggressive angiosarcomas in aP2-Cre mice. Whereas, the conditional deletion of Tsc1 leads to vascular tumor formation that mimic human kaposiform hemangioendotheliomas. We used Affymetrix microarrays to compare angiosarcoma tumors with the combined activation of KrasG12D and loss of Cdkn2a and the conditional deletion of Tsc1
Project description:Comparison of aP2-Cre and Cdh5-Cre tdTomato labaled cells from hindlimb and SCM muscles shows they mark the same endothelial cell populations
Project description:Gene expression of tdTomato positive FACS isolated sternocleidomastoid msucles from aP2-Cre;R26tdTomato and Cdh5-Cre;R26tdTomato animals for ssGSEA analysis
Project description:Comparision of aP2-Cre, Myog-Cre and Cdh5-Cre SmoM2 driven tumors shows that Cdh5-Cre tumors express muscle markers but have unique gene expression profiles; Comparision of aP2-Cre SmoM2/tdTomato tumor cells and Cdh5-Cre SmoM2/tdTomato tumor cells isolated by FACS for CD31-/tdTomato+ cells reveals that Cdh5-Cre tumors are not rhabdomyosarcoma
Project description:Comparision of aP2-Cre, Myog-Cre and Cdh5-Cre SmoM2 driven tumors shows that Cdh5-Cre tumors express muscle markers but have unique gene expression profiles Comparision of aP2-Cre SmoM2/tdTomato tumor cells and Cdh5-Cre SmoM2/tdTomato tumor cells isolated by FACS for CD31-/tdTomato+ cells reveals that Cdh5-Cre tumors are not rhabdomyosarcoma
Project description:Comparision of aP2-Cre, Myog-Cre and Cdh5-Cre SmoM2 driven tumors shows that Cdh5-Cre tumors express muscle markers but have unique gene expression profiles Comparision of aP2-Cre SmoM2/tdTomato tumor cells and Cdh5-Cre SmoM2/tdTomato tumor cells isolated by FACS for CD31-/tdTomato+ cells reveals that Cdh5-Cre tumors are not rhabdomyosarcoma
Project description:Lung cancers exhibit pronounced functional heterogeneity, confounding precision medicine. We studied how the cell-of-origin contributes to phenotypic heterogeneity following conditional expression of KrasG12D and loss of Lkb1 (Kras;Lkb1). Using progenitor cell type-restricted adenoviral-Cre to target cells expressing Surfactant Protein C (SPC) or club cell antigen 10 (CC10), we show that Ad5-CC10-Cre infected mice exhibit a shorter latency compared with Ad5-SPC-Cre cohorts. We further demonstrate that CC10+ cells are the predominant progenitors of adenosquamous carcinoma (ASC) tumors, and give rise to a wider spectrum of histotypes that includes mucinous and acinar adenocarcinomas. Transcriptome analysis shows ASC histotype-specific upregulation of proinflammatory and immunomodulatory genes. This is accompanied with an ASC-specific immunosuppressive environment, consisting of downregulated MHC genes, recruitment of CD11b+ Gr-1+ tumor-associated neutrophils (TANs) and decreased T-cell numbers. We conclude that progenitor cell-specific etiology influences the Kras;Lkb1-driven tumor histopathology spectrum and histotype-specific immune microenvironment.
Project description:Sporozoite-specific genes were induced in parasites that express AP2-O whose AP2 domain was swapped with that of AP2-Sp. DNA construct encoding AP2 domain of AP2-Sp was introduced into P. bergei schizonts. It was integrated into the locus of the endogenious AP2-O gene, resulting in swap of the AP2-domain (AP2-O::Sp parasites). Gene expression was analyzed in ookinetes cultured for 20h.