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Effect of enhanced early life nutrition on testes miRNA transcriptome in bull calves

ABSTRACT: Effect of enhanced early life nutrition on testes miRNA transcriptome in bull calves

PROVIDER: PRJNA798827 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17672005.fastq.gz	Fastqsanger.gz
	SRR17672006.fastq.gz	Fastqsanger.gz
	SRR17672007.fastq.gz	Fastqsanger.gz
	SRR17672008.fastq.gz	Fastqsanger.gz
	SRR17672009.fastq.gz	Fastqsanger.gz

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Effect of enhanced early life nutrition on testes miRNA transcriptome in bull calves

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Project description:It has been established that enhanced early life nutrition progresses sexual development in the bull calf through neuroendocrine signalling via the hypothalamic-pituitary-testicular axis. However, the underlying molecular mechanisms regulating this process have not been fully elucidated. This study measured the impact of contrasting feeding regimes in the first 12 wk of life, known to impact age at puberty, on the proteomic landscape of the testes of bull calves. Holstein bull calves with a mean (±SD) bodyweight and age of 48.8 (± 5.3) kg and 17.5 (± 2.8) days, were designated to high (HI; n=10) or moderate (MOD; n=10) dietary groups, with diets designed to provoke growth rates of 1.0 and 0.5 kg/day, respectively. At 12 wk of age, all calves were euthanized, and testes parenchyma harvested. HI calves were heavier at slaughter (112.4 v 88.7 (2.98) kg, P<0.001), and had a greater average daily gain (ADG) of (0.88 v 0.58 kg, P<0.001). The turquoise network from the protein analyses contained the protein CDH13 which is involved in testes development. Gene ontology analysis of the turquoise network revealed enrichment of genes with functions related to cholesterol biosynthesis, IGF-1 signalling, insulin receptor/secretion signalling, androgen signalling and Sertoli cell junction signalling.

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Project description:Pre-pubertal Holstein bull calves fed a higher plane of nutrition had larger testes, earlier puberty, higher serum LH, testosterone and greater sperm production potential than those fed a restricted diet. In addition, pre-pubertal calves fed a high-nutrition diet had higher IGF-I and more proliferating and differentiating Sertoli cells much earlier in life, compared to those fed normal or low-nutrition diets. The objective was to determine changes in mRNA expression of genes in the testes of bulls fed either a high or low pre-pubertal diet. Holstein bull calves maintained on either a high (20% crude protein (CP) and 71.6% Total Digestible Nutrients (TDN)) or low (12% CP and 64.4% TDN) diet from 2 wk of age, were castrated at 8, 16, 24 and 32 wk and testicular mRNA extracted and sequenced. Differential expression of genes mainly occurred at 16 and 24 wk, with minor changes detected at 32 wk. At 16 wks, functional analysis of DE mRNA with DAVID revealed the common biological processes enriched to be "cholesterol" and "fatty acid biosynthesis" with majority of the genes including HMGCR, HMGCS1, HSD17 being upregulated in high-diet bulls (P<0.05). Major pathways enriched at 16 wks were "cholesterol biosynthesis", "steroid metabolism" and "activation of gene expression by Sterol regulatory element-binding protein (SREBP)" (P<0.05). Mature Sertoli cell marker Connexin 43, was upregulated at 16 wk, whereas an immature Sertoli cell marker, AMH was downregulated at 24 wk, in the high-diet group. Network analysis using IPA, revealed an indirect interaction between insulin family receptor and most upregulated cholesterol biosynthesis genes, implying regulation of testicular function. Thus, enhanced pre-pubertal nutrition in Holstein bulls enhanced testicular cholesterol/steroid biosynthesis and Sertoli cell maturation to promote early reproductive development.

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