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ABSTRACT: Transcriptome of jejunal tissue in neonatal goats subjected to delaying colostrum feeding

PROVIDER: PRJNA799403 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17698147_1.fastq.gz	Fastqsanger.gz
	SRR17698147_2.fastq.gz	Fastqsanger.gz
	SRR17698148_1.fastq.gz	Fastqsanger.gz
	SRR17698148_2.fastq.gz	Fastqsanger.gz
	SRR17698149_1.fastq.gz	Fastqsanger.gz

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Project description:miRNA sequencing of jejunal tissues in neonatal goats subjected to delayed colostrum feeding

| PRJNA799441 | ENA

Genome wide transcriptome analysis provides bases on colonic mucosal immune system development in neonatal calves affected by colostrum feeding strategies in neonatal calves

Project description:Delivery of colostrum within the first several hours after birth is vital for establishing successful passive immunity in neonatal dairy calves. However, it is unclear whether the difference in colostrum feeding strategy can affect the development of the calf gastrointestinal tract. The aim of this study was to evaluate the effect of colostrum feeding time within the first 12 h after birth on the colonic mucosal immune system in neonatal calves using a genome wide transcriptome analysis.RNA sequencing based transcriptome analysis of colon tissues collected from twenty-seven male Holstein calves which were randomly assigned to one of three colostrum feeding strategies (immediately after birth (TRT0); 6 h after birth (TRT6); 12 h after birth (TRT12)) and were euthanized at 51 h of age detected 15935 ± 210, 15332 ± 415, and 15539 ± 440 expressed genes in groups, respectively. The core transcriptome of the colon in dairy calves included 12,678 genes, with enriched “cellular process” and “metabolic process” as the top three biological functions. Expression of 802 immune related genes were detected in the colon tissue. Principal component analysis of the transcriptomes did not display a clear separation by colostrum feeding strategy, and differential abundance analyses showed no significant difference in the expression of immune related genes among the treatments.Transcriptome analysis indicates that the development of the colonic mucosal immune system in neonatal calves may be independent of the timing of initial colostrum meal within 12 h after birth.

2018-08-09 | GSE113264 | GEO

Early microbial colonisation affects transcriptome of pig jejunum perfused with E. coli F4, F4 fimbria or Lact. amylovorus

Project description:An early settlement of a complex gut microbiota can protect against gastro-intestinal dysbiosis, but the effects of neonatal microbiota colonization on the gut barrier upon the further encounter of favorable bacteria or not, are largely unknown. The jejunal transcriptome of differently perfused intestinal loops of 12 caesarian-derived pigs previously associated with microbiota of different complexity was studied. Pigs received pasteurized sow colostrum at birth (d0), 2 mL of starter microbiota (10^7 CFU of each Lactob. Amylovorus (LAM), Clostr. glycolicum, and Parabacteroides spp.) on d1-d3 of age and either a placebo inoculant (simple association, SA) or an inoculant consisting of diluted feces of an adult sow (complex association, CA) on d3-d4 of age. On days 26-37 of age, jejunal loops were perfused for 8 h with either enterotoxigenic E. coli F4 (ETEC), F4 fimbriae (F4), LAM or saline (CTRL) and jejunal samples were obtained from each piglet immediately afterwards. The analysis of whole mRNA transcript expression was done by AffymetrixÂ©Porcine Gene 1.1 ST array strips, a Robust Multichip Analysis was performed on the CEL files, and Transcripts IDâs were associated to 13,406 Human gene names, based on Sus scrofa Ensemble

2016-02-11 | E-GEOD-77787 | biostudies-arrayexpress

Early microbial colonisation affects transcriptome of pig jejunum perfused with E. coli F4, F4 fimbria or Lact. amylovorus

2016-02-11 | GSE77787 | GEO

Delayed colostrum feeding influences ileum transcriptome profile in neonatal calves

Project description:Delayed colostrum feeding influences ileum transcriptome profile in neonatal calves

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Delayed colostrum feeding influences ileum transcriptome profile in neonatal calves

Project description:Our study would like to explore the different colostrum feeding time treatment, as well as the influence of host-microbial interaction on transcriptome profile and enriched functions of the two day old dairy calves.

2021-03-05 | GSE113331 | GEO

Project description:16s rRNA sequence data of jejunal digesta in neonatal goats

| PRJNA799234 | ENA

Project description:Pasteurized Colostrum Feeding

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Project description:Ileal Transcriptome of neonatal goats

| PRJNA782168 | ENA

Defining Age- and Lactocrine-Sensitive Elements of the Neonatal Porcine Uterine microRNA-mRNA Interactome

Project description:Factors delivered to offspring in colostrum within two days of birth support neonatal porcine uterine development. The uterine mRNA transcriptome is affected by age and nursing during this period. Whether uterine microRNA (miRNA) expression is affected similarly is unknown. Objectives were to: 1) determine effects of age and nursing on porcine uterine miRNA expression between birth and postnatal day (PND) 2 using small RNA sequencing (smRNA-Seq) and; 2) define affected miRNA-mRNA interactions and associated biological processes using integrated target prediction analysis.

2017-02-16 | GSE89414 | GEO

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