Project description:We conducted comparative genome hybridization experiments to catalogue the common copy number variation evident between 269 individuals from three geographically distinct human populations (Yoruban, Chinese/Japanese, European) against a single reference individual.
Project description:We used 10× Genomics single-cell RNA sequencing to investigate the cellular and transcriptional mechanisms underlying sperm motility differences in goose testes. Spermatogenesis depends on the coordinated interaction between germ cells and somatic cells in the testicular microenvironment, but the cellular basis of sperm motility variation remains unclear in geese. Here, we generated a single-cell transcriptomic atlas of testes from high- and low-sperm-motility groups and identified the major germ-cell and somatic-cell populations involved in spermatogenesis. These findings provide new insights into the molecular regulation of avian spermatogenesis and establish a valuable resource for understanding the mechanisms underlying male fertility in geese.
Project description:To understand the molecular basis of distinct pork quality in Chinese indigenous and Western breed, longissimus dorsi samples were collected from three adult Northeastern Indigenous and from three adult Large White. Total RNA was extracted and subjected to porcine Affymetrix Genechip. The study helps to elucidate the genetic mechnism of divergent pork quality and provide the theory basis for selection and genetic improvement of meat quality traits in porcine.
2016-09-02 | GSE24192 | GEO
Project description:An intercross population study identifies crucial genes associated with economically important traits in domestic geese
| PRJNA1043749 | ENA
Project description:An intercross population study identifies crucial genes associated with economically important traits in domestic geese
Project description:In order to efficiently breed plants for climate change, it is critical to identify traits in seedlings that are effective predictors of agronomically important traits at maturity that could be used to screen lines. Our work shows that the response of circadian clock to changes in photoperiod in seedling Arabidopsis is predictive of bolting time and bolting-associated traits, including the degree of synchronization in bolting. Using the first ever Recombinant Inbred Lines (RILs) between African and European Arabidopsis lineages, we find distinct Quantitative Trait Loci (QTLs) associated both with the synchronicity of circadian responses to photoperiodic changes and with bolting-related traits. Two QTLs contain K-Homology Domain RNA binding proteins (KH17 and KH29) associated with splicing variants in the MADS AFFECTING FLOWERING2 and 3 (MAF2, MAF3) genes, including generating chimeric transcripts between these two adjacent genes containing the MADS-box of MAF2 and the K-box dimerisation domain of MAF3. Many of the variants in KH17 in Arabidopsis ecotypes are found within its prion-like domain and are associated with de-coupling the mean and synchronicity of flowering time. Therefore, we have identified novel potential mechanisms that link synchronised responses on a diurnal scale and a developmental scale.
Project description:Domesticated animals all show the same patterns regarding phenotypic traits and behaviour, collectively known as the domestic phenotype. All domestic chicken come from the red junglefowl. By keeping three separate populations of junglefowl and selecting for high, low or intermediate fear responses towards humans, the goal is to in the low fear group start to unlock domestic phenotypes.
